Department of Internal Medicine, Allergy and Clinical Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Department of Neurology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Egypt J Immunol. 2022 Jul;29(3):80-89.
Stroke is long known to be followed by a series of immunosuppressive events, and infections might be a cause of death after an acute insult of stroke. The aim of our work was to assess the percentage of neutrophils showing spontaneous oxidative burst in patients with acute ischemic stroke. The study included 30 patients with acute cerebral infarction subjected to the following: magnetic resonance imaging of the brain immediately on admission, and blood sampling on day one of admission (baseline) and after 3 days of admission. Blood samples were used for the assessment of: differential leucocyte count and percentage of neutrophils showing spontaneous oxidative burst, performed by flow cytometry. Thirty age and gender matched controls were also recruited. Neutrophil respiratory burst percentage was significantly lower in stroke patients in comparison to controls (P < 0.001), and stroke patients had significantly lower neutrophil respiratory burst percent on day 3 of admission compared to the baseline (P < 0.001). Stroke-induced immune alterations including impairment of the first-line defense performed by neutrophils against bacteria. The hypothesis that these changes enhance susceptibility to acquired infections is supported by our observation that oxidative burst in neutrophils was more impaired in patients with stroke who exhibited subsequent stroke-associated infections.
中风后已知会发生一系列免疫抑制事件，感染可能是中风急性损伤后的死亡原因。我们的工作目的是评估急性缺血性中风患者中性粒细胞自发氧化爆发的比例。该研究纳入了 30 名急性脑梗死患者，进行了以下检查：入院时立即进行脑部磁共振成像，以及入院第 1 天（基线）和第 3 天入院时的血液采样。血液样本用于评估：通过流式细胞术进行的白细胞分类计数和自发氧化爆发的中性粒细胞百分比。还招募了 30 名年龄和性别匹配的对照者。与对照组相比，中风患者的中性粒细胞呼吸爆发百分比明显降低（P < 0.001），与基线相比，中风患者在入院第 3 天的中性粒细胞呼吸爆发百分比明显降低（P < 0.001）。中风引起的免疫改变包括中性粒细胞对细菌的一线防御功能受损。我们的观察结果支持了这样一种假设，即这些变化增强了获得性感染的易感性，即随后发生中风相关感染的患者中性粒细胞的氧化爆发受损更为严重。
