Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, College of Life Science and Technology, Tarim University, Alar, Xinjiang, China.
College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China.
Trop Anim Health Prod. 2022 Jun 27;54(4):218. doi: 10.1007/s11250-022-03216-5.
The effects of androgens on human skin mainly include the regulation of growth and differentiation of hair follicles and sebaceous glands. Androgens may have some physiological roles in sheep skin that are similar to those of humans, but further confirmation is needed. Therefore, Hetian sheep were chosen in this study as an animal model to explore the effects of testosterone on skin structure and factors related to androgen conversion and binding in Hetian sheep. The sheep were treated with different concentrations of testosterone for 42 days. Skin tissue sections were prepared and then subjected to hematoxylin-eosin, Sacpic, Masson's trichrome, and Oil Red O staining to observe changes in skin morphology. Changes in the content of blood-related factors were also detected using ELISA kits. The skin tissue distribution of androgen receptor was explored by immunohistochemistry and immunofluorescence assays. The results showed that testosterone significantly increases the sebaceous gland area and stimulates the formation of new sebaceous glands. Further exploration revealed that testosterone promotes the proliferation of sebaceous gland juvenile cells. However, testosterone was found to have no significant effect on hair follicle density and hair follicle structure. Testosterone increased dihydrotestosterone levels but decreased 5α-reductase 1 and 5α-reductase 2 levels. The androgen receptors were distributed in the hair follicles, sebaceous glands, and some major skin appendages of Hetian sheep. This study suggests that androgens can be effective in regulating sebum production in sheep. This study will help advance research efforts to further explore the molecular and cellular mechanisms by which androgens modify sheep follicles and sebaceous glands.
雄激素对人类皮肤的影响主要包括调节毛囊和皮脂腺的生长和分化。雄激素在绵羊皮肤中可能具有一些与人类相似的生理作用，但需要进一步证实。因此，本研究选择和田羊作为动物模型，探讨睾酮对和田羊皮肤结构及相关雄激素转化和结合因子的影响。将绵羊用不同浓度的睾酮处理 42 天。制备皮肤组织切片，然后进行苏木精-伊红、萨皮克、马松三色和油红 O 染色，观察皮肤形态的变化。还使用 ELISA 试剂盒检测血液相关因子含量的变化。通过免疫组织化学和免疫荧光分析探讨雄激素受体在皮肤组织中的分布。结果表明，睾酮显著增加了皮脂腺面积并刺激了新的皮脂腺形成。进一步的探索表明，睾酮促进了皮脂腺幼年细胞的增殖。然而，睾酮对毛囊密度和毛囊结构没有明显影响。睾酮增加了二氢睾酮水平，但降低了 5α-还原酶 1 和 5α-还原酶 2 的水平。雄激素受体分布在和田羊的毛囊、皮脂腺和一些主要皮肤附属器中。本研究表明，雄激素可有效调节绵羊皮脂的产生。本研究将有助于推进研究工作，进一步探讨雄激素修饰绵羊毛囊和皮脂腺的分子和细胞机制。
