Terry Fox Laboratory, BC Cancer Agency, Vancouver, Canada; Department of Medical Genetics, University of British Columbia, Vancouver Canada.
Terry Fox Laboratory, BC Cancer Agency, Vancouver, Canada.
J Biol Chem. 2022 Aug;298(8):102199. doi: 10.1016/j.jbc.2022.102199. Epub 2022 Jun 24.
The nucleus is a highly organized organelle with an intricate substructure of chromatin, RNAs, and proteins. This environment represents a challenge for maintaining protein quality control, since non-native proteins may interact inappropriately with other macromolecules and thus interfere with their function. Maintaining a healthy nuclear proteome becomes imperative during times of stress, such as upon DNA damage, heat shock, or starvation, when the proteome must be remodeled to effect cell survival. This is accomplished with the help of nuclear-specific chaperones, degradation pathways, and specialized structures known as protein quality control (PQC) sites that sequester proteins to help rapidly remodel the nuclear proteome. In this review, we focus on the current knowledge of PQC sites in Saccharomyces cerevisiae, particularly on a specialized nuclear PQC site called the intranuclear quality control site, a poorly understood nuclear inclusion that coordinates dynamic proteome triage decisions in yeast.
核是一个高度组织化的细胞器，具有复杂的染色质、RNA 和蛋白质亚结构。这种环境对维持蛋白质质量控制构成了挑战，因为非天然蛋白质可能与其他大分子不适当相互作用，从而干扰其功能。在应激时，如 DNA 损伤、热休克或饥饿时，维持健康的核蛋白质组变得至关重要，此时必须重塑蛋白质组以促进细胞存活。这是通过核特异性伴侣、降解途径以及称为蛋白质质量控制 (PQC) 位点的专门结构来完成的，这些结构可以隔离蛋白质，以帮助快速重塑核蛋白质组。在这篇综述中，我们重点介绍了酿酒酵母中 PQC 位点的最新知识，特别是一个专门的核 PQC 位点，称为核内质量控制位点，这是一个了解甚少的核内含物，它协调酵母中动态蛋白质组分类决策。
