Ningxia Medical University, Yinchuan, 750004, China.
Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Yinchuan, 750004, China.
BMC Bioinformatics. 2023 May 31;24(1):222. doi: 10.1186/s12859-023-05353-6.
To explore dermatomyositis signature genes as potential biomarkers of hepatocellular carcinoma and their associated molecular regulatory mechanisms.
Based on the mRNA-Seq data of dermatomyositis and hepatocellular carcinoma in public databases, five dermatomyositis signature genes were screened by LASSO regression analysis and support vector machine (SVM) algorithm, and their biological functions in dermatomyositis with hepatocellular carcinoma were investigated, and a nomogram risk prediction model for hepatocellular carcinoma was constructed and its predictive efficiency was initially evaluated. The immune profile in hepatocellular carcinoma was examined based on the CIBERSORT and ssGSEA algorithms, and the correlation between five dermatomyositis signature genes and tumor immune cell infiltration and immune checkpoints in hepatocellular carcinoma was investigated.
The expression levels of five dermatomyositis signature genes were significantly altered in hepatocellular carcinoma and showed good diagnostic efficacy for hepatocellular carcinoma, suggesting that they may be potential predictive targets for hepatocellular carcinoma, and the risk prediction model based on five dermatomyositis signature genes showed good risk prediction efficacy for hepatocellular carcinoma and has good potential for clinical application. In addition, we also found that the upregulation of SPP1 expression may activate the PI3K/ART signaling pathway through integrin-mediated activation, which in turn regulates the development and progression of hepatocellular carcinoma.
LY6E, IFITM1, GADD45A, MT1M, and SPP1 are potential predictive targets for new-onset hepatocellular carcinoma in patients with dermatomyositis, and the upregulation of SPP1 expression may activate the PI3K/ART signaling pathway through the mediation of integrins to promote the development and progression of hepatocellular carcinoma.
探索皮肌炎特征基因作为肝细胞癌潜在生物标志物及其相关分子调控机制。
基于公共数据库中皮肌炎和肝细胞癌的 mRNA-Seq 数据，通过 LASSO 回归分析和支持向量机（SVM）算法筛选出 5 个皮肌炎特征基因，研究其在伴发肝细胞癌的皮肌炎中的生物学功能，并构建肝细胞癌的列线图风险预测模型，初步评估其预测效能。基于 CIBERSORT 和 ssGSEA 算法检测肝细胞癌的免疫图谱，研究 5 个皮肌炎特征基因与肝细胞癌肿瘤免疫细胞浸润和免疫检查点的相关性。
5 个皮肌炎特征基因在肝细胞癌中的表达水平明显改变，对肝细胞癌具有良好的诊断效能，提示其可能是肝细胞癌的潜在预测靶点，基于 5 个皮肌炎特征基因的风险预测模型对肝细胞癌具有良好的风险预测效能，具有良好的临床应用潜力。此外，我们还发现 SPP1 表达的上调可能通过整合素介导的激活激活 PI3K/ART 信号通路，进而调节肝细胞癌的发生和发展。
LY6E、IFITM1、GADD45A、MT1M 和 SPP1 是皮肌炎患者新发肝细胞癌的潜在预测靶点，SPP1 表达的上调可能通过整合素的介导激活 PI3K/ART 信号通路，促进肝细胞癌的发生和发展。
