Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China; School of Public Health, Zhejiang Chinese Medical University, Hangzhou 310053, PR China.
Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China; School of Public Health, Zhejiang Chinese Medical University, Hangzhou 310053, PR China.
Food Res Int. 2022 Jul;157:111259. doi: 10.1016/j.foodres.2022.111259. Epub 2022 Apr 16.
This present study was designed to test the protective role of two Lactobacillus plantarum strains, E680 and ZY08, against alcoholic liver disease (ALD) in C57BL/6 mice. The ALD mouse model was established by exposing the mice to a Lieber-DeCarli ethanol liquid diet. The two probiotic strains (10 cfu/day) were administered by oral gavage, respectively. Our data showed that L. plantarum ZY08, but not E680, intervention significantly mitigated alcohol-related hepatic steatosis, liver injury, intestinal barrier, and it alleviated plasma endotoxin (LPS) levels, and affected hepatic genes relating to lipid metabolism. Furthermore, Lactobacillus plantarum ZY08 effectively restored intestinal flora homeostasis via recovering flora abundance, including Blautia, Oscillibacter, Lachnoclostridium and Intestimonas, and consequently elevated intestinalshort-chain fatty acid (SCFA) content. More importantly, removing intestinal microorganisms through ABX gavage markedly abolished the beneficial aspects of Lactobacillus plantarum ZY08, indicating that the regulative role of Lactobacillus plantarum ZY08 contributed to its protective role against ALD. Overall, Lactobacillus plantarum ZY08 is a potential candidate for mitigating alcohol-induced hepatic steatosis and liver injury.
本研究旨在测试两株植物乳杆菌（E680 和 ZY08）对 C57BL/6 小鼠酒精性肝病（ALD）的保护作用。通过给小鼠喂食 Lieber-DeCarli 乙醇液体饮食来建立 ALD 小鼠模型。通过口服灌胃分别给予两种益生菌株（10 cfu/天）。我们的数据表明，植物乳杆菌 ZY08 干预而非 E680 干预显著减轻了酒精相关的肝脂肪变性、肝损伤、肠道屏障，并降低了血浆内毒素（LPS）水平，影响了与脂质代谢相关的肝脏基因。此外，植物乳杆菌 ZY08 通过恢复菌群丰度（包括 Blautia、Oscillibacter、Lachnoclostridium 和 Intestimonas）有效恢复了肠道菌群平衡，从而提高了肠道短链脂肪酸（SCFA）含量。更重要的是，通过 ABX 灌胃去除肠道微生物显著消除了植物乳杆菌 ZY08 的有益作用，表明植物乳杆菌 ZY08 的调节作用有助于其预防 ALD 的作用。总的来说，植物乳杆菌 ZY08 是一种减轻酒精诱导的肝脂肪变性和肝损伤的潜在候选物。
