Zaki Asmaa Hussein, Haiying Bao, Zhijun Li
Key Laboratory of Edible Fungi Resources and Utilization, Ministry of Agriculture and Rural Affairs, Jilin Agricultural University, Changchun 130118, Jilin, China.
College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, Jilin, China.
Evid Based Complement Alternat Med. 2022 Jun 18;2022:5762847. doi: 10.1155/2022/5762847. eCollection 2022.
is a known medicinal mushroom in China. It possesses many biological activities. This study investigated the effect of . petroleum ether and water extracts (200, 500, and 1,000 mg/kg BW) on mammary gland differentiation during lactation. However, prolactin, growth hormone, progesterone, and estrogen levels were determined in serum by ELISA assay. Immunofluorescence, western blot, and real-time PCR were utilized to evaluate the expression levels of -casein, -Lactalbumin, prolactin receptor, progesterone receptor, and STAT-5a. The immunohistochemistry staining was used to detect the presence of steroid receptors. The results showed that petroleum ether and water extracts increased milk yield and milk content of calcium, total fat, total carbohydrate, and total protein. Prolactin and growth hormone levels were significantly upregulated in all treated groups compared with the control group. In contrast, progesterone and estrogen were downregulated. The high doses of petroleum ether and water extracts increased the expression levels of -Cas, -Lactalb, PRLR, PR, and STAT-5a. The observation of histological sections showed that the extracts induced higher mammary gland differentiation than the control group. This study is the first to use mushrooms as nutritional supplements to improve milk production and mammary gland differentiation during lactation.
在中国，它是一种已知的药用蘑菇。它具有多种生物活性。本研究调查了石油醚提取物和水提取物（200、500和1000毫克/千克体重）对哺乳期乳腺分化的影响。然而，通过酶联免疫吸附测定法测定血清中的催乳素、生长激素、孕酮和雌激素水平。利用免疫荧光、蛋白质印迹法和实时聚合酶链反应来评估β-酪蛋白、α-乳白蛋白、催乳素受体、孕酮受体和信号转导及转录激活因子5a（STAT-5a）的表达水平。免疫组织化学染色用于检测类固醇受体的存在。结果表明，石油醚提取物和水提取物提高了产奶量以及牛奶中钙、总脂肪、总碳水化合物和总蛋白的含量。与对照组相比，所有处理组的催乳素和生长激素水平均显著上调。相比之下，孕酮和雌激素水平下调。高剂量的石油醚提取物和水提取物提高了β-酪蛋白、α-乳白蛋白、催乳素受体、孕酮受体和信号转导及转录激活因子5a的表达水平。组织学切片观察表明，提取物诱导的乳腺分化高于对照组。本研究首次使用蘑菇作为营养补充剂来提高哺乳期的产奶量和乳腺分化。
