Key Laboratory of Medicinal Fungal Resources and Development and Utilization, Jilin Agricultural University, Changchun, China.
Medical Academy, Changchun Science-Technology University, Changchun, China.
J Food Biochem. 2021 Apr;45(4):e13514. doi: 10.1111/jfbc.13514. Epub 2021 Feb 11.
A response surface method was used to optimize the extraction of polysaccharides from Leucocalocybe mongolica. Moreover, the preliminary structural characteristics and antitumor activity of L. mongolica polysaccharide (LMP) were investigated. The results showed that the optimized extraction technological parameters for LMP were 93°C extraction temperature, 5 hr extraction time, and 30 ml/g liquid-to-solid ratio. The LMP content extracted under the optimal conditions was 6.64%. LC-MS/MS results indicated that LMP is a neutral polysaccharide composed of d-fructose, d-mannose, dextrose anhydrate, d-xylose, trehalose, and galactose. The tumor inhibition rate was significantly improved by LMP treatment. LMP had minimal toxicity based on the significant decrease in AST and BUN levels; VEGF protein levels were also significantly decreased. In contrast, the levels of IFN-γ, IL-2, IL-6, and TNF-α were improved. The results of ELISA, H&E staining, TUNEL assay, immunohistochemistry, and western blotting indicated that the LMP exhibited antitumor activity in vivo by promoting apoptosis, mediating inflammatory responses, and inhibiting angiogenesis. PRACTICAL APPLICATIONS: As one of the main bioactive components, fungal polysaccharide has always been a hot research topic. Fungal polysaccharides are carbohydrate polymers composed of monosaccharide units bound together by glycosidic linkages, which have been found to be involved in many biological processes. In this research, the LMP structure was analyzed, and the immunohistochemical and western blot analysis confirmed that, LMP could effectively reduce the generation of tumor angiogenesis, promote apoptosis of tumor cell sand inhibit tumor growth. The results of this study can effectively provide a basis for clinical research and development of antitumor drugs, and lay a foundation for the study of the antitumor effects of wild edible and medicinal fungi.
采用响应面法优化了从蒙古口蘑中提取多糖的工艺。此外，还研究了蒙古口蘑多糖（LMP）的初步结构特征和抗肿瘤活性。结果表明，LMP 的最佳提取工艺参数为：提取温度 93℃，提取时间 5 小时，液固比 30ml/g。在最佳条件下提取的 LMP 含量为 6.64%。LC-MS/MS 结果表明，LMP 是一种由 d-果糖、d-甘露糖、葡萄糖无水物、d-木糖、海藻糖和半乳糖组成的中性多糖。LMP 处理可显著提高肿瘤抑制率。基于 AST 和 BUN 水平的显著降低，LMP 的毒性最小；VEGF 蛋白水平也显著降低。相反，IFN-γ、IL-2、IL-6 和 TNF-α 的水平得到改善。ELISA、H&E 染色、TUNEL 检测、免疫组化和 Western blot 结果表明，LMP 通过促进细胞凋亡、介导炎症反应和抑制血管生成，在体内发挥抗肿瘤活性。实际应用：作为主要生物活性成分之一，真菌多糖一直是研究热点。真菌多糖是由单糖单元通过糖苷键结合而成的碳水化合物聚合物，已发现其参与多种生物过程。在这项研究中，分析了 LMP 的结构，并通过免疫组化和 Western blot 分析证实，LMP 可以有效减少肿瘤血管生成的产生，促进肿瘤细胞凋亡并抑制肿瘤生长。本研究结果可为抗肿瘤药物的临床研究和开发提供有效依据，为野生食用药用真菌的抗肿瘤作用研究奠定基础。
