pH 响应型葡萄糖胺锚定的聚多巴胺包覆的介孔硅纳米粒子用于安德森型多金属氧酸盐在乳腺癌中的递送。
pH-responsive glucosamine anchored polydopamine coated mesoporous silica nanoparticles for delivery of Anderson-type polyoxomolybdate in breast cancer.
机构信息
Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Ph.D student of Medicinal chemistry, Department of Medicinal Chemistry, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
J Microencapsul. 2022 Aug;39(5):433-451. doi: 10.1080/02652048.2022.2096139. Epub 2022 Jul 13.
AIM
This study aimed to develop novel pH-sensitive Glucosamine (Glu) targeted Polydopamine (PDA) coated mesoporous silica (SBA-15) nanoparticles (NPs) for selective delivery of anticancer Anderson-type manganese polyoxomolybdate (POMo) to breast cancer.
METHODS
The POMo@SBA-PDA-Glu NPs were prepared via direct hydrothermal synthesis of SBA, POMo loading, in situ PDA post functionalization, and Glu anchoring; the chemical structures were fully studied by different characterisation methods. The anticancer activity was studied by MTT method and Annexin V-FITC apoptosis detection kit.
RESULTS
The optimised NPs had a hydrodynamic size (HS) of 195 nm, a zeta potential (ZP) of -18.9 mV, a loading content percent (LC%) of 45%, and a pH-responsive release profile. The targeted NPs showed increased anticancer activity against breast cancer cell lines compared to the free POMo with the highest cellular uptake and apoptosis level in the MDA-MB-231 cells.
CONCLUSIONS
POMo@SBA-PDA-Glu NPs could be a promising anticancer candidate for further studies.
目的
本研究旨在开发新型 pH 敏感的葡萄糖胺（Glu）靶向聚多巴胺（PDA）包覆的介孔硅（SBA-15）纳米粒子（NPs），用于将抗癌安德森型锰多金属氧酸盐（POMo）选择性递送至乳腺癌。
方法
通过 SBA 的直接水热合成、POMo 负载、原位 PDA 后功能化和 Glu 锚定，制备了 POMo@SBA-PDA-Glu NPs；通过多种表征方法充分研究了化学结构。通过 MTT 法和 Annexin V-FITC 凋亡检测试剂盒研究了抗癌活性。
结果
优化后的 NPs 的水动力直径（HS）为 195nm，Zeta 电位（ZP）为-18.9mV，载药含量百分比（LC%）为 45%，具有 pH 响应释放特性。与游离 POMo 相比，靶向 NPs 对乳腺癌细胞系表现出更高的抗癌活性，在 MDA-MB-231 细胞中具有最高的细胞摄取率和凋亡水平。
结论
POMo@SBA-PDA-Glu NPs 可能是进一步研究的有前途的抗癌候选物。
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