Loginov A S, Bendikov E A, Kel'nia Zh A, Petrakov A V
Ter Arkh. 1987;59(2):84-9.
Hydroxylase and demethylase activity of cytochrome P450-dependent liver monooxygenases was estimated in healthy persons and patients with chronic diseases of the hepatobiliary system. Metabolite urine content and 4-hydroxyantipyrine and norantipyrine clearance were measured within 24 h after oral antipyrine administration (10 mg/kg). It was shown that progressive antipyrine metabolic derangements depended on the form and stage of chronic diffuse liver lesion, which was especially marked in patients with chronic active viral hepatitis, liver cirrhosis with or without ascites and with signs of encephalopathy. More considerable inhibition of N-demethylase monooxygenase activity as compared to hydroxylase activity in patients with chronic active viral hepatitis showed different sensitivity of some cytochrome P450 forms to pathogenetic factors. It could be of diagnostic value. The authors put forward the idea of interaction between the oxygenase and immune systems in drug detoxication in liver diseases.
在健康人和患有肝胆系统慢性疾病的患者中，评估了细胞色素P450依赖性肝单加氧酶的羟化酶和脱甲基酶活性。在口服安替比林（10mg/kg）后24小时内，测量代谢产物尿含量以及4-羟基安替比林和去甲安替比林清除率。结果表明，安替比林代谢紊乱的进展取决于慢性弥漫性肝损伤的形式和阶段，这在慢性活动性病毒性肝炎、伴有或不伴有腹水以及有脑病迹象的肝硬化患者中尤为明显。与慢性活动性病毒性肝炎患者的羟化酶活性相比，N-脱甲基酶单加氧酶活性受到更显著的抑制，这表明某些细胞色素P450形式对致病因素具有不同的敏感性。这可能具有诊断价值。作者提出了在肝脏疾病药物解毒过程中加氧酶和免疫系统之间相互作用的观点。
