Abdel-Haleem Fatehy M, Rizk Mahmoud S, El-Beshlawy Menna M
Chemistry Department, Faculty of Science, Cairo University Giza Egypt
Center for Hazards Mitigation, Environmental Studies and Research (CHMESR), Cairo University Giza Egypt.
RSC Adv. 2022 Jun 15;12(27):17645-17654. doi: 10.1039/d2ra02064e. eCollection 2022 Jun 7.
IVH may be administered orally twice per day for treating heart failure, sinus rhythm, heart-related chest pain and angina pectoris, and its overdose may cause problems such as blurred vision, uncontrolled blood pressure, prolonged bradycardia, and others. A molecularly imprinted polymer-based bulk optode, miptode, was constructed for the determination of ivabradine hydrochloride (IVH) in its pharmaceutical preparation Procoralan®. The molecularly-imprinted polymers (MIPs) were prepared in different ratios, and MIP3 had the highest imprinting factor (1.6) as an ionophore in the miptode preparation. The miptode was prepared using weight ratios of 30% PVC polymer, 62% nitrophenyl octyl ether (NPOE) plasticizer, 6% MIP3 ionophore, 1% tetraphenyl borate derivative (TPB) ion-exchanger, and 1% ETH7075 chromoionophore; this miptode exhibited an absorbance increase at 530 nm in the concentration range of 10-10 M with a detection limit of 3.1 μM using Tris-HCl buffer of pH 7.2. The miptode was imaged using AFM, and showed the dissolution of all components except MIP particles which exhibited restricted solubility. However, the incorporation of MIP3 as an ionophore improved the selectivity coefficient over the interfering species that may exist in the pharmaceutical formulation to an extent that was not reported before; coefficients of IVH over sodium, magnesium, and glucose were improved by 5, 4 and 2 orders, when compared to the previous sensor that operated with the molecular interaction mechanism. The selectivity improvement in miptode is due to the Key-Lock fitting (host-guest molecular recognition) between the MIP particles and the template IVH molecule which is transduced with the ion-exchange process of the chromoionophore. The miptode has a response time of 1-2 minutes, and a reliable lifetime of two months. The miptode was applied successfully for the determination of IVH in the pharmaceutical preparation Procoralan® with recovery values of 89-99.8% with low standard deviations of <1.2.
伊伐布雷定可每日口服两次，用于治疗心力衰竭、窦性心律、心脏相关胸痛和心绞痛，过量服用可能会导致视力模糊、血压失控、心动过缓延长等问题。构建了一种基于分子印迹聚合物的整体光极（miptode），用于测定其药物制剂可兰特（Procoralan®）中的盐酸伊伐布雷定（IVH）。以不同比例制备分子印迹聚合物（MIP），其中MIP3作为miptode制剂中的离子载体，具有最高的印迹因子（1.6）。使用30%聚氯乙烯聚合物、62%硝基苯基辛醚（NPOE）增塑剂、6% MIP3离子载体、1%四苯基硼酸盐衍生物（TPB）离子交换剂和1% ETH7075发色离子载体的重量比制备miptode；该miptode在pH 7.2的Tris-HCl缓冲液中，在10⁻¹⁰ M的浓度范围内，于530 nm处吸光度增加，检测限为3.1 μM。使用原子力显微镜对miptode进行成像，结果显示除MIP颗粒外所有组分均溶解，而MIP颗粒溶解度受限。然而，将MIP3作为离子载体的引入，使对药物制剂中可能存在的干扰物质的选择性系数提高到了前所未有的程度；与之前基于分子相互作用机制运行的传感器相比，IVH对钠、镁和葡萄糖的选择性系数分别提高了5个、4个和2个数量级。miptode的选择性提高归因于MIP颗粒与模板IVH分子之间的“锁钥”适配（主客体分子识别），并通过发色离子载体的离子交换过程进行传导。miptode的响应时间为1 - 2分钟，可靠寿命为两个月。该miptode成功应用于药物制剂可兰特（Procoralan®）中IVH的测定，回收率为89 - 99.8%，标准偏差低至<1.2。
