Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, Huainan, Anhui, China.
Anhui Province Engineering Laboratory of Occupational Health and Safety, Huainan, Anhui, China.
PeerJ. 2022 Jun 23;10:e13632. doi: 10.7717/peerj.13632. eCollection 2022.
Coal workers' pneumoconiosis (CWP) is an occupational disease that severely damages the life and health of miners. However, little is known about the molecular and cellular mechanisms changes associated with lung inflammation and fibrosis induced by coal dust. As a non-destructive technique for measuring biological tissue, confocal Raman spectroscopy provides accurate molecular fingerprints of label-free tissues and cells. Here, the progression of lung inflammation and fibrosis in a murine model of CWP was evaluated using confocal Raman spectroscopy.
A mouse model of CWP was constructed and biochemical analysis in lungs exposed to coal dust after 1 month (CWP-1M) and 3 months (CWP-3M) control tissues (NS) were used by confocal Raman spectroscopy. H&E, immunohistochemical and collagen staining were used to evaluate the histopathology alterations in the lung tissues.
The CWP murine model was successfully constructed, and the mouse lung tissues showed progression of inflammation and fibrosis, accompanied by changes in NF-κB, p53, Bax, and Ki67. Meanwhile, significant differences in Raman bands were observed among the different groups, particularly changes at 1,248, 1,448, 1,572, and 746 cm. These changes were consistent with collagen, Ki67, and Bax levels in the CWP and NS groups.
Confocal Raman spectroscopy represented a novel approach to the identification of the biochemical changes in CWP lungs and provides potential biomarkers of inflammation and fibrosis.
煤工尘肺（CWP）是一种严重损害矿工生命和健康的职业病。然而，对于煤尘引起的肺部炎症和纤维化的分子和细胞机制变化知之甚少。共聚焦拉曼光谱作为一种用于测量生物组织的非破坏性技术，可提供无标记组织和细胞的准确分子指纹。在这里，使用共聚焦拉曼光谱评估了 CWP 小鼠模型中肺部炎症和纤维化的进展。
构建 CWP 小鼠模型，并使用共聚焦拉曼光谱分析暴露于煤尘 1 个月（CWP-1M）和 3 个月（CWP-3M）后的肺部组织（NS）的生化变化。通过 H&E、免疫组织化学和胶原染色评估肺组织的组织病理学变化。
成功构建了 CWP 小鼠模型，小鼠肺组织显示出炎症和纤维化的进展，同时伴随着 NF-κB、p53、Bax 和 Ki67 的变化。同时，不同组之间观察到 Raman 带的显著差异，特别是在 1248、1448、1572 和 746cm 处的变化。这些变化与 CWP 和 NS 组中的胶原、Ki67 和 Bax 水平一致。
共聚焦拉曼光谱代表了一种识别 CWP 肺部生化变化的新方法，并提供了炎症和纤维化的潜在生物标志物。
