Lee Jaehee, Park Leejin, Kim Hyeyoung, Rho Bong-Il, Han Rafael Taeho, Kim Sewon, Kim Hee Jin, Na Heung Sik, Back Seung Keun
Neuroscience Research Institute and Department of Physiology, Korea University College of Medicine, Seoul 02841, Korea.
Glovi Plastic Surgery, Seoul 06031, Korea.
Korean J Physiol Pharmacol. 2022 Jul 1;26(4):287-295. doi: 10.4196/kjpp.2022.26.4.287.
() is known to induce apoptosis of host immune cells and impair phagocytic clearance, thereby being pivotal in the pathogenesis of atopic dermatitis (AD). Adipose-derived stem cells (ASCs) exert therapeutic effects against inflammatory and immune diseases. In the present study, we investigated whether systemic administration of ASCs restores the phagocytic activity of peripheral blood mononuclear cells (PBMCs) and decolonizes cutaneous under AD conditions. AD was induced by injecting capsaicin into neonatal rat pups. ASCs were extracted from the subcutaneous adipose tissues of naïve rats and administered to AD rats once a week for a month. Systemic administration of ASCs ameliorated AD-like symptoms, such as dermatitis scores, serum IgE, IFN-γ/IL-4 cell ratio, and skin colonization by in AD rats. Increased FasL mRNA and annexin V/7-AAD cells in the PBMCs obtained from AD rats were drastically reversed when co-cultured with ASCs. In contrast, both PBMCs and CD163 cells bearing fluorescent zymosan particles significantly increased in AD rats treated with ASCs. Additionally, the administration of ASCs led to an increase in the mRNA levels of antimicrobial peptides, such as cathelicidin and β-defensin, in the skin of AD rats. Our results demonstrate that systemic administration of ASCs led to decolonization of by attenuating apoptosis of immune cells in addition to restoring phagocytic activity. This contributes to the improvement of skin conditions in AD rats. Therefore, administration of ASCs may be helpful in the treatment of patients with intractable AD.
已知（某物质）可诱导宿主免疫细胞凋亡并损害吞噬清除功能，因此在特应性皮炎（AD）的发病机制中起关键作用。脂肪干细胞（ASC）对炎症和免疫疾病具有治疗作用。在本研究中，我们调查了全身给予ASC是否能恢复外周血单核细胞（PBMC）的吞噬活性，并使AD条件下皮肤中的（某种微生物）去定植。通过向新生大鼠幼崽注射辣椒素诱导AD。从未处理大鼠的皮下脂肪组织中提取ASC，并每周一次给予AD大鼠，持续一个月。全身给予ASC改善了AD样症状，如AD大鼠的皮炎评分、血清IgE、IFN-γ/IL-4细胞比例以及皮肤中的（某种微生物）定植情况。当与ASC共培养时，从AD大鼠获得的PBMC中增加的FasL mRNA和膜联蛋白V/7-AAD细胞显著逆转。相反，在接受ASC治疗的AD大鼠中，携带荧光酵母聚糖颗粒的PBMC和CD163细胞均显著增加。此外，给予ASC导致AD大鼠皮肤中抗菌肽（如cathelicidin和β-防御素）的mRNA水平升高。我们的结果表明，全身给予ASC除了恢复吞噬活性外，还通过减弱免疫细胞凋亡导致（某种微生物）去定植。这有助于改善AD大鼠的皮肤状况。因此，给予ASC可能有助于治疗难治性AD患者。
