Department of Radiation Oncology, Huai'an Hospital Affiliated to Xuzhou Medical University, Huai'an City, Jiangsu Province, China.
Department of Radiation Oncology, The Comprehensive Cancer Centre of Nanjing Drum Tower Hospital, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China.
J Cancer Res Clin Oncol. 2023 Jun;149(6):2543-2550. doi: 10.1007/s00432-022-04122-x. Epub 2022 Jun 29.
Vascular endothelial growth factor (VEGF) is related to the radiation resistance of tumors, resulting in the failure of tumor radiotherapy. The purpose of this study was to discuss the role of VEGF in radiotherapy resistance of esophageal squamous cell carcinoma (ESCC). We used the VEGF kit by ELISA to detect the serum VEGF level of ESCC patients who only received radiotherapy. The expression of VEGF in ESCC cells after siRNA treatment was verified by Western blot. The sensitivity of ESCC cells to radiation after knocking down VEGF was analyzed by Clonogenic assay and Cell counting kit (CCK-8). The results showed that the level of serum VEGF in patients with ESCC before and after radiotherapy was related to the clinical response, and it was confirmed that knocking down the expression of VEGF in ESCC cells improved the sensitivity to radiation.
血管内皮生长因子（VEGF）与肿瘤的辐射抗性有关，导致肿瘤放疗失败。本研究旨在探讨 VEGF 在食管鳞癌（ESCC）放疗抵抗中的作用。我们使用 ELISA 试剂盒检测仅接受放疗的 ESCC 患者的血清 VEGF 水平。通过 Western blot 验证 ESCC 细胞经 siRNA 处理后 VEGF 的表达。通过集落形成实验和 CCK-8 分析敲低 VEGF 后 ESCC 细胞对辐射的敏感性。结果表明，放疗前后 ESCC 患者血清 VEGF 水平与临床反应相关，证实敲低 ESCC 细胞中 VEGF 的表达可提高其对辐射的敏感性。
