Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA.
Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA.
STAR Protoc. 2022 Jun 17;3(3):101478. doi: 10.1016/j.xpro.2022.101478. eCollection 2022 Sep 16.
Assembloids generated from human pluripotent stem cells are self-organizing, multicellular models that recapitulate aspects of cell-cell interactions and circuit assembly during neural development Here, we present protocols to functionally monitor, in forebrain assembloids, the migration of GABAergic interneurons from the ventral to the dorsal forebrain and the activity in early cortical networks. Specifically, we describe high-resolution imaging and analysis of neuronal migration as well as calcium imaging of network activity in forebrain assembloids. For complete details on the use and execution of this protocol, please refer to Birey et al. (2022).
从人类多能干细胞生成的类器官是自我组织的多细胞模型，可再现神经发育过程中细胞-细胞相互作用和回路组装的某些方面。在此，我们提供了在大脑前脑类器官中功能监测 GABA 能中间神经元从腹侧向背侧大脑前脑迁移以及早期皮质网络活动的方案。具体来说，我们描述了对神经元迁移的高分辨率成像和分析，以及大脑前脑类器官中网络活动的钙成像。如需使用和执行此方案的完整详细信息，请参考 Birey 等人（2022 年）。
