Department of Medicine, University of California, San Diego, La Jolla, CA, USA.
Veterans Affairs San Diego Health Care System, La Jolla, CA, USA.
Methods Mol Biol. 2022;2506:167-186. doi: 10.1007/978-1-0716-2364-0_12.
ILC2s are key players in the emergence of type 2 inflammation in many pulmonary diseases. While several phenotypic markers can be used to identify ILC2s, our method utilizes the surface markers CD127 and ST2 to classify a group of type 2 cytokine-producing ILC2s upon activation by the fungal allergen Alternaria alternata . Here, we provide our protocol for the detection and isolation of a highly pure population of pulmonary mouse ILCs via flow cytometry and cell sorting. We also describe the methods for in vitro stimulation to assess the functionality of ILC2s.
ILC2s 是许多肺部疾病中 2 型炎症出现的关键因素。虽然有几种表型标志物可用于鉴定 ILC2s，但我们的方法利用表面标志物 CD127 和 ST2，在真菌过敏原 Alternaria alternata 激活后，对一群产生 2 型细胞因子的 ILC2s 进行分类。在这里，我们提供了一种通过流式细胞术和细胞分选检测和分离高度纯化的小鼠肺 ILC 群体的方案。我们还描述了体外刺激评估 ILC2 功能的方法。
