Biological Science Program, Department of Biological and Environmental Sciences, College of Art and Science, Qatar University, P.O. Box 2713, Doha, Qatar.
Environmental Science Centre, Qatar University, P.O. Box 2713, Doha, Qatar.
Sci Rep. 2022 Jun 30;12(1):11012. doi: 10.1038/s41598-022-14871-x.
Mango (Mangifera indica) is the second most internationally traded tropical fruit in the world. The fruit has high nutritional value. Its susceptibility to postharvest diseases and chill injuries increases its storage cost and put stress on exploring natural products that can increase its shelf-life. Our team has previously described Prosopis juliflora water-soluble leaf ethanolic (PJ-WS-LE) extract with fungicidal effectiveness against spoiling fungi. The present study explores P. juliflora genetic diversity in the state of Qatar and the antifungal effectiveness of the leaf extract of plants collected from different locations. The study also evaluates PJ-WS-LE extract efficacy against Alternaria. alternata and Colletotrichum gloeosporioides inoculated in mango samples and the power of the extract as coating material. P. juliflora samples collected from six different locations showed genetic and antimicrobial effectiveness similarities. They showed also similarity to the sequence representing P. juliflora 18S ribosomal RNA partial sequence, accession number JX139107.1 originated from India. PJ-WS-LE extract (8 mg/ml) has 80% efficacy in controlling A. alternata in mango and it lowers C. gloeosporioides disease severity by 53.4%. PJ-WS-LE extract (8 mg/ml) embedded in 1% chitosan maintained mango quality for 5 weeks. In vivo results of PJ-WS-LE extract highlights the potentials of the extract as chemical fungicides replacement.
芒果（Mangifera indica）是世界上第二大国际贸易热带水果。这种水果具有很高的营养价值。它容易受到采后疾病和冷害的影响，增加了其储存成本，并促使人们探索可以延长其货架期的天然产品。我们的团队之前曾描述过具有杀菌作用的普纳菊水溶性叶乙醇（PJ-WS-LE）提取物，可以有效抵抗腐烂真菌。本研究探讨了卡塔尔境内普纳菊的遗传多样性，以及从不同地点采集的植物叶片提取物的抗真菌效果。该研究还评估了 PJ-WS-LE 提取物对芒果样本中接种的链格孢和胶孢炭疽菌的功效，以及提取物作为涂层材料的能力。从六个不同地点采集的普纳菊样本表现出遗传和抗菌效果的相似性。它们与代表印度的 P. juliflora 18S 核糖体 RNA 部分序列的序列（登录号 JX139107.1）也相似。PJ-WS-LE 提取物（8mg/ml）对芒果中的Alternaria. alternata 有 80%的抑制作用，可使胶孢炭疽菌的病情严重程度降低 53.4%。1%壳聚糖中嵌入的 PJ-WS-LE 提取物可使芒果的质量保持 5 周。PJ-WS-LE 提取物的体内结果突出了其作为化学杀菌剂替代品的潜力。
