Kumari V B Chandana, Huligere Sujay S, Ramu Ramith, Naik Bajpe Shrisha, Sreenivasa M Y, Silina Ekaterina, Stupin Victor, Achar Raghu Ram
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysore, India.
Department of Biotechnology, Sri Dharmasthala Manjunatheshwara College (Autonomous), Ujire, India.
Front Microbiol. 2022 Jun 14;13:911243. doi: 10.3389/fmicb.2022.911243. eCollection 2022.
Fermented foods are sources of functionally salient microbes. These microbes when ingested can regulate biomolecule metabolism which has a plethora of health benefits. Lactic acid bacteria species (LABs) isolated from fermented beetroot were biochemically characterized and validated using 16s rRNA sequence. Also, an assay was conducted to confirm the probiotic activity of the isolates. The cell-free supernatant (CS), cell-free extract (CE), and intact cell (IC) were evaluated for α-glucosidase and α-amylase inhibition. The six isolates RAMULAB01-06 were categorized to be spp. by observing phenotypic and biochemical characters. Molecular validation using 16S rDNA sequencing, followed by homology search in NCBI database, suggested that the isolates are >95% similar to and . Also, isolates exhibited probiotic potential with a high survival rate (>96%) in the gastrointestinal condition, and adherence capability (>53%), colonization (>86%), antibacterial, and antibiotic activity. The safety assessments expressed that the isolates are safe. The α-glucosidase and α-amylase inhibition by CS, CE, and IC ranged from 3.97 ± 1.42% to 53.91 ± 3.11% and 5.1 ± 0.08% to 57.15 ± 0.56%, respectively. Hence, these species have exceptional antidiabetic potential which could be explicated to its use as a functional food and health-related food products.
发酵食品是功能显著的微生物来源。这些微生物在摄入后可调节生物分子代谢，对健康有诸多益处。对从发酵甜菜根中分离出的乳酸菌进行了生化特性鉴定，并使用16s rRNA序列进行了验证。此外，还进行了一项试验以确认分离株的益生菌活性。对无细胞上清液（CS）、无细胞提取物（CE）和完整细胞（IC）进行了α-葡萄糖苷酶和α-淀粉酶抑制作用评估。通过观察表型和生化特征，将六个分离株RAMULAB01 - 06归类为 spp.。使用16S rDNA测序进行分子验证，随后在NCBI数据库中进行同源性搜索，结果表明这些分离株与 和 的相似度>95%。此外，分离株在胃肠道条件下表现出较高的存活率（>96%）、黏附能力（>53%）、定植能力（>86%）、抗菌和抗生素活性等益生菌潜力。安全性评估表明这些分离株是安全的。CS、CE和IC对α-葡萄糖苷酶和α-淀粉酶的抑制率分别为3.97±1.42%至53.91±3.11%和5.1±0.08%至57.15±0.56%。因此，这些菌种具有卓越的抗糖尿病潜力，这可以解释为其可用于功能性食品和与健康相关的食品产品。
