Kwun Se Young, Bae Young Woo, Yoon Jeong Ah, Park Eun Hee, Kim Myoung Dong
Division of Food Biotechnology and Biosystems Engineering, Kangwon National University, Chuncheon, 24341 Korea.
Food Sci Biotechnol. 2020 Jun 2;29(8):1125-1130. doi: 10.1007/s10068-020-00760-4. eCollection 2020 Aug.
In this study, lactic acid bacteria strains (LABs) were isolated from Korean traditional fermented food and examined as potential probiotics using in vitro methods. Ten LAB strains survived in de Man, Rogosa and Sharpe broth adjusted to pH 2.5 were tested for resistance to acidic conditions and bile, antimicrobial activity, and α-glucosidase inhibitory activity. Among them, strain MBEL1397 showed antimicrobial activity against and exhibited survival rates of over 97% in acidic and bile conditions. The α-glucosidase inhibitory activity was 3.91 ± 0.25%, corresponding to approximately 2.3 times higher than that of acarbose. MBEL1397 was susceptible to ampicillin, erythromycin, and penicillin G and identified as It was deposited to Korean Collection for Type Culture (KCTC) as KCTC14037BP. In conclusion, these results demonstrate that MBEL1397 might be prominent probiotics with potential hypoglycemic effects.
在本研究中，从韩国传统发酵食品中分离出乳酸菌菌株（LABs），并使用体外方法将其作为潜在益生菌进行检测。对在pH 2.5的德氏、罗氏和夏普肉汤中存活的10株LAB菌株进行了耐酸性和胆汁性、抗菌活性以及α-葡萄糖苷酶抑制活性的测试。其中，MBEL1397菌株对[具体对象未给出]具有抗菌活性，并且在酸性和胆汁条件下的存活率超过97%。α-葡萄糖苷酶抑制活性为3.91±0.25%，约为阿卡波糖的2.3倍。MBEL1397对氨苄青霉素、红霉素和青霉素G敏感，并被鉴定为[具体菌种未给出]。它作为KCTC14037BP保藏于韩国典型培养物保藏中心（KCTC）。总之，这些结果表明MBEL1397可能是具有潜在降血糖作用的优质益生菌。
