Li Baiyun, Li Hui, Xu Zehua, Guo Xinnian, Zhou Tao, Shi Jiangli
Horticulture Institute, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, China.
Institute of Agricultural Resources and Environment, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, China.
Front Genet. 2022 Jun 14;13:863746. doi: 10.3389/fgene.2022.863746. eCollection 2022.
The early ripening jujube is an immensely popular fresh fruit due to its high commercial value as well as rich nutrition. However, little is known about the mechanism of jujube fruit's ripening. In this study, the transcriptome profiles were comprehensively analyzed between the 'Lingwu Changzao' jujube and its early-ripening mutant during the fruit development and maturity. A total of 5,376 and 762 differentially expressed genes (DEGs) were presented at 80 and 90 days after the flowering of the jujube fruit, respectively. Furthermore, 521 common DEGs were identified as candidate genes that might be associated with the fruit's early ripening. Our findings demonstrated that in a non-climacteric jujube fruit, abscisic acid (ABA) was more greatly involved in fruit ripening than ethylene. Meanwhile, the fruit ripening of the early-ripening mutant was regulated by eight promotors of DEGs related to glucose and fructose, seven repressors of DEGs related to brassinosteroid signal transduction, and a series of transcription factor genes (MYB, Bhlh, and ERF). Additionally, the expression of 20 candidate DEGs was further validated by real-time PCR during the late fruit maturation stage. Collectively, the present study sheds light on the metabolic mechanism of the fruit's early ripening and provides valuable candidate genes for the early-ripening mutant's breeding.
早酥枣因其较高的商业价值和丰富的营养成分而成为广受欢迎的新鲜水果。然而，关于枣果实成熟的机制却知之甚少。在本研究中，对‘灵武长枣’及其早熟突变体在果实发育和成熟过程中的转录组图谱进行了全面分析。在枣果开花后80天和90天，分别共呈现出5376个和762个差异表达基因（DEGs）。此外，521个共同的差异表达基因被鉴定为可能与果实早熟相关的候选基因。我们的研究结果表明，在非跃变型枣果实中，脱落酸（ABA）比乙烯在果实成熟过程中发挥的作用更大。同时，早熟突变体的果实成熟受与葡萄糖和果糖相关的差异表达基因的8个启动子、与油菜素内酯信号转导相关的差异表达基因的7个抑制子以及一系列转录因子基因（MYB、Bhlh和ERF）调控。此外，在果实成熟后期通过实时荧光定量PCR进一步验证了20个候选差异表达基因的表达。总体而言，本研究揭示了果实早熟的代谢机制，并为早熟突变体的育种提供了有价值的候选基因。
