Bioengineering Laboratory, Department of Textile and Fiber Engineering, Indian Institute of Technology, New Delhi 110016, India.
Sikkim Manipal Institute of Medical Sciences, Tadong, Gangtok, Sikkim 737102, India.
Int J Biol Macromol. 2022 Sep 1;216:303-311. doi: 10.1016/j.ijbiomac.2022.06.168. Epub 2022 Jun 28.
Present study aims at developing antimicrobial cotton gauze by dip coating of sodium alginate (SA), glycerol (Gly) and tannic acid (TA) blend. SA blends were prepared with varying concentration of glycerol in the range of 10-40 %. Blended films were fabricated and characterized by Fourier transform-infrared (FTIR) spectroscopy, X-ray diffraction (XRD), tensile studies, and contact angle analysis. The mechanical behavior of films indicated significant decrease in the tensile strength and modulus with the increase in the glycerol content due to the plasticization effect. The hydrophilicity of the blend films increased with increase in the glycerol content. TA was added to the blend as an antimicrobial agent. These blends were coated on the cotton gauze by dip coating method and their characterizations were carried out by the scanning electron microscopy (SEM) which revealed a smooth coating of SA:Gly:TA blend on cotton gauze. Antimicrobial analysis of TA coated gauzes was carried out which showed >95 % viable colony reduction against E. coli and S. aureus. Cytocompatibility studies indicated excellent cell-compatible activity. These results implicated that such coated gauzes are promising candidate that hold the great potential to be utilized as infection-resistant material in the health care sector.
本研究旨在通过海藻酸钠(SA)、甘油(Gly)和单宁酸(TA)混合液的浸涂来制备抗菌棉纱布。SA 混合液中甘油的浓度范围为 10-40%。通过傅里叶变换红外(FTIR)光谱、X 射线衍射(XRD)、拉伸研究和接触角分析对共混膜进行了表征。由于增塑作用,薄膜的力学性能表明随着甘油含量的增加,拉伸强度和模量显著降低。共混膜的亲水性随甘油含量的增加而增加。TA 被添加到共混物中作为抗菌剂。这些共混物通过浸涂法涂覆在棉纱布上,通过扫描电子显微镜(SEM)对其进行了表征,结果表明 SA:Gly:TA 共混物在棉纱布上形成了光滑的涂层。对 TA 涂层纱布进行了抗菌分析,结果表明对大肠杆菌和金黄色葡萄球菌的活菌菌落减少率超过 95%。细胞相容性研究表明具有极好的细胞相容性。这些结果表明,这种涂层纱布是一种很有前途的候选材料,有望在医疗保健领域用作抗感染材料。