Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 11, 87-100 Torun, Poland.
Institute of Polymers, Composites and Biomaterials, National Research Council, v.le J.F. Kennedy 54, Mostra d'OLtremare Pad. 20, 80125 Naples, Italy.
ACS Appl Mater Interfaces. 2024 Sep 4;16(35):46102-46112. doi: 10.1021/acsami.4c09909. Epub 2024 Aug 20.
Wound healing is a dynamic process that requires an optimal extracellular environment, as well as an accurate synchronization between various cell types. Over the past few years, great efforts have been devoted to developing novel approaches for treating and managing burn injuries, sepsis, and chronic or accidental skin injuries. Multifunctional smart-polymer-based dressings represent a promising approach to support natural healing and address several problems plaguing partially healed injuries, including severe inflammation, scarring, and wound infection. Naturally derived compounds offer unique advantages such as minimal toxicity, cost-effectiveness, and outstanding biocompatibility along with potential anti-inflammatory and antimicrobial activity. Herein, the main driving idea of the work was the design and development of konjac glucomannan d-glucono-1,5-lactone (KG) films bioactivated by tannic acid and d-glucono-1,5-lactone (GL) addition. Our analysis, using attenuated total reflectance-Fourier transform infrared, atomic force microscopy, and surface energy measurements demonstrated that tannic acid (TA) clearly interacted with the KG matrix, acting as its cross-linker, whereas GL was embedded within the polymer structure. All developed films maintained a moist environment, which represents a pivotal property for wound dressing. Hemocompatibility experiments showed that all tested films exhibited no hemolytic impact on human erythrocytes. Moreover, the presence of TA and GL enhanced the metabolic and energetic activity in human dermal fibroblasts, as indicated by the MTT assay, showing results exceeding 150%. Finally, all films demonstrated high antibacterial properties as they significantly reduced the multiplication rate of both and in bacterial broth and created the inhibition zones for in agar plates. These remarkable outcomes make the KG/TA/GL film promising candidates for wound healing applications.
伤口愈合是一个动态的过程，需要一个最佳的细胞外环境，以及各种细胞类型之间的精确同步。在过去的几年中，人们致力于开发治疗和管理烧伤、脓毒症和慢性或意外皮肤损伤的新方法。基于多功能智能聚合物的敷料是一种很有前途的方法，可以支持自然愈合，并解决部分愈合损伤所面临的几个问题，包括严重的炎症、瘢痕形成和伤口感染。天然衍生化合物具有独特的优势，如最小的毒性、成本效益和出色的生物相容性，以及潜在的抗炎和抗菌活性。在此，这项工作的主要设计理念是设计和开发经单宁酸和 d-葡萄糖酸-1,5-内酯（GL）处理而生物活化的魔芋葡甘聚糖 d-葡萄糖酸-1,5-内酯（KG）薄膜。我们的分析，使用衰减全反射傅里叶变换红外光谱、原子力显微镜和表面能测量，表明单宁酸（TA）与 KG 基质明显相互作用，作为其交联剂，而 GL 则嵌入聚合物结构中。所有开发的薄膜都保持湿润的环境，这是伤口敷料的关键特性。血液相容性实验表明，所有测试的薄膜对人红细胞均无溶血影响。此外，TA 和 GL 的存在增强了人皮肤成纤维细胞的代谢和能量活性，MTT 测定结果超过 150%。最后，所有薄膜都表现出很高的抗菌性能，因为它们显著降低了细菌肉汤中 和 的倍增率，并在琼脂平板上为 创造了抑制区。这些显著的结果使 KG/TA/GL 薄膜成为伤口愈合应用的有前途的候选材料。
