环状 RNA circSEPT9 在子宫内膜癌中上调，并通过甲基化下调 miR-186 促进细胞侵袭和迁移。

Circular RNA circSEPT9 Is Upregulated in Endometrial Cancer and Promotes Cell Invasion and Migration by Downregulating miR-186 through Methylation.

机构信息

Department of Gynecology, Shunde Hospital of Southern Medical University, Foshan, Guangdong, China

Department of Gynecology, Shunde Hospital of Southern Medical University, Foshan, Guangdong, China.

出版信息

Ann Clin Lab Sci. 2022 May;52(3):399-405.

PMID:35777791

Abstract

OBJECTIVE

MicroRNA-186 (miR-186) and circular RNA (circRNA) circSEPT9 are two crucial players in cancer biology, while their roles in endometrial cancer (EC) are unclear. Our preliminary sequencing analysis revealed the potential crosstalk between circSEPT9 and miR-186 in EC.

MATERIALS AND METHODS

The expression levels of circSEPT9 and miR-186 in EC and paired non-tumor tissues from 64 EC patients were detected by RT-qPCRs. Correlation analysis was carried out with Pearson's correlation coefficient. The role of circSEPT9 in regulating the expression of miR-186 and the methylation of miR-186 gene was explored by RT-qPCR and methylation specific PCR (MSP), respectively. Cell invasion and migration were evaluated by Transwell assays.

RESULTS

CircSEPT9 was upregulated in EC, and miR-186 was downregulated in EC. MiR-186 and circSEPT9 were inversely correlated across EC tissue samples, but not non-tumor samples. Overexpression of circSEPT9 decreased the expression levels of miR-186 and increased the methylation of miR-186 gene. CircSEPT9 increased cell invasion and migration and suppressed the role of miR-186 in inhibiting cell behaviors.

CONCLUSION

Therefore, circSEPT9 is upregulated in EC and promotes cell invasion and migration by downregulating miR-186 through methylation.

摘要

目的

微小 RNA-186（miR-186）和环状 RNA（circRNA）circSEPT9 是癌症生物学中的两个关键分子，但其在子宫内膜癌（EC）中的作用尚不清楚。我们的初步测序分析显示，circSEPT9 和 miR-186 之间可能存在相互作用。

材料和方法

通过 RT-qPCR 检测 64 例 EC 患者的 EC 和配对非肿瘤组织中 circSEPT9 和 miR-186 的表达水平。采用 Pearson 相关系数进行相关性分析。通过 RT-qPCR 和甲基化特异性 PCR（MSP）分别探讨 circSEPT9 对 miR-186 表达和 miR-186 基因甲基化的调控作用。通过 Transwell 实验评估细胞侵袭和迁移。

结果

circSEPT9 在 EC 中上调，miR-186 在 EC 中下调。miR-186 和 circSEPT9 在 EC 组织样本中呈负相关，但在非肿瘤样本中无相关性。circSEPT9 的过表达降低了 miR-186 的表达水平，并增加了 miR-186 基因的甲基化。circSEPT9 增加了细胞侵袭和迁移，并抑制了 miR-186 抑制细胞行为的作用。