Department of Obstetrics and Gynecology, Nanjing Gulou Hospital, Nanjing City, Jiangsu Province, 210009, P.R. China.
Zibo Central Hospital, Zibo City, Shandong Province, 255000, P.R. China.
Crit Rev Eukaryot Gene Expr. 2022;32(4):41-47. doi: 10.1615/CritRevEukaryotGeneExpr.2022039647.
It is known that the circular RNA (circRNA) molecule circRIMS is overexpressed in gastric cancer and plays an oncogenic role. However, its role in other cancers is unknown. In this study, we analyzed its role in endometrial cancer (EC). EC and paired non-tumor tissue samples were collected from a total of 63 EC patients and subjected to total RNA isolations and reverse transcription quantitative polymerase chain reaction (RT-qPCR) to analyze the differential expression of circRIMS and miR-505. Overexpression of circRIMS and miR-505 was reached in EC cells and their interaction was analyzed using RT-qPCRs. The role of circRIMS in regulating miR-505 methylation was analyzed by methylation-specific RT-qPCR. Bromodeoxyuridine (BrdU) assay was performed to analyze the roles of circRIMS and miR-505 in regulating cell proliferation. circRIMS was upregulated in EC, while miR-505 was downregulated in EC. circRIMS and miR-505 were inversely correlated across both EC and non-tumor tissues. In EC cells, circRIMS overexpression decreased miR-505 expression and increased miR-505 gene methylation. BrdU assay showed that circRIMS overexpression increased cell proliferation and reduced the inhibitory effects of miR-505 overexpression on cell proliferation. circRIMS may downregulate miR-505 through methylation to increase cell proliferation.
已知环状 RNA (circRNA) 分子 circRIMS 在胃癌中过表达，发挥致癌作用。然而，其在其他癌症中的作用尚不清楚。在本研究中，我们分析了 circRIMS 在子宫内膜癌 (EC) 中的作用。从 63 名 EC 患者中收集 EC 和配对非肿瘤组织样本，进行总 RNA 分离和逆转录定量聚合酶链反应 (RT-qPCR)，以分析 circRIMS 和 miR-505 的差异表达。通过 RT-qPCR 分析 EC 细胞中 circRIMS 和 miR-505 的过表达及其相互作用。通过甲基化特异性 RT-qPCR 分析 circRIMS 调节 miR-505 甲基化的作用。通过溴脱氧尿苷 (BrdU) 测定分析 circRIMS 和 miR-505 在调节细胞增殖中的作用。EC 中 circRIMS 上调，而 miR-505 在 EC 中下调。EC 和非肿瘤组织中 circRIMS 和 miR-505 呈负相关。在 EC 细胞中，circRIMS 过表达降低了 miR-505 的表达并增加了 miR-505 基因的甲基化。BrdU 测定表明，circRIMS 过表达增加了细胞增殖，并降低了 miR-505 过表达对细胞增殖的抑制作用。circRIMS 可能通过甲基化下调 miR-505 以增加细胞增殖。
