Yang Pengxia, Yun Kunlun, Zhang Ruying
Department of Obstetrics and Gynecology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot City, 010050, Inner Mongolia, People's Republic of China.
Physical Examination Center, Inner Mongolia Autonomous Region People's Hospital, Hohhot City, 010050, Inner Mongolia, People's Republic of China.
Mamm Genome. 2021 Dec;32(6):488-494. doi: 10.1007/s00335-021-09899-9. Epub 2021 Aug 18.
It has been reported that circ-ATAD1 promotes the development of gastric cancer. This study was carried out to analyze the role of circ-ATAD1 in endometrial cancer (EC). EC and paired non-tumor tissues from EC patients (n = 60) were subjected to the isolation of total RNA and RT-qPCR to analyze the expression of circ-ATAD1 and miR-10a. Correlations between circ-ATAD1 and miR-10a were analyzed by Pearson's correlation coefficient. The effects of circ-ATAD1 overexpression on miR-10a expression and methylation were analyzed by RT-qPCR and methylation-specific PCR (MSP). The roles of circ-ATAD1 and miR-10a in regulating EC cell invasion and migration were analyzed by Transwell assays. We found that circ-ATAD1 was downregulated in EC, while miR-10a was upregulated in EC. An inverse correlation between circ-ATAD1 and miR-10a was observed across EC samples. In EC cells, circ-ATAD1 overexpression decreased miR-10a expression and increased miR-10a gene methylation. Transwell assay analysis showed that circ-ATAD1 overexpression totally reversed the enhancing effects of miR-10a on EC cell invasion and migration. Circ-ATAD1 is downregulated in EC and may suppress EC cell invasion and migration by downregulating miR-10a through methylation.
据报道，环状ATAD1促进胃癌的发展。本研究旨在分析环状ATAD1在子宫内膜癌（EC）中的作用。对60例EC患者的EC组织及配对的非肿瘤组织进行总RNA分离和RT-qPCR，以分析环状ATAD1和miR-10a的表达。通过Pearson相关系数分析环状ATAD1与miR-10a之间的相关性。通过RT-qPCR和甲基化特异性PCR（MSP）分析环状ATAD1过表达对miR-10a表达和甲基化的影响。通过Transwell实验分析环状ATAD1和miR-10a在调节EC细胞侵袭和迁移中的作用。我们发现环状ATAD1在EC中表达下调，而miR-10a在EC中表达上调。在EC样本中观察到环状ATAD1与miR-10a呈负相关。在EC细胞中，环状ATAD1过表达降低了miR-10a的表达并增加了miR-10a基因的甲基化。Transwell实验分析表明，环状ATAD1过表达完全逆转了miR-10a对EC细胞侵袭和迁移的增强作用。环状ATAD1在EC中表达下调，可能通过甲基化下调miR-10a来抑制EC细胞的侵袭和迁移。
