Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Department of Radiotherapy, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Hepatobiliary Pancreat Dis Int. 2022 Aug;21(4):370-377. doi: 10.1016/j.hbpd.2022.06.009. Epub 2022 Jun 23.
ATP-binding cassette transporter G1 (ABCG1) regulates cellular cholesterol homeostasis and plays a significant role in tumor immunity. But, for hepatocellular carcinoma (HCC), the role of ABCG1 has not been investigated. Thus, the aim of this study was to evaluate the prognostic value and clinicopathological significance of ABCG1 in HCC.
One hundred and four adult patients with HCC were enrolled, and ABCG1 expression in paired HCC specimens was determined by immunohistochemistry. All these patients were stratified by ABCG1 expression, Kaplan-Meier analysis was used to compare the overall survival (OS) and recurrence-free survival (RFS), and Cox regression analysis was used to determine independent predictors of tumor recurrence.
Upregulation of ABCG1 was observed in HCC samples compared to matched tumor-adjacent tissues. Patients with high nuclear ABCG1 expression had lower OS and RFS (P = 0.012 and P = 0.020, respectively). High nuclear ABCG1 expression was related to larger tumor size (P = 0.004) and tumor recurrence (P = 0.027). Although ABCG1 was expressed in the cytoplasm, cytosolic expression could not predict the outcome in patients with HCC. A new stratification pattern was established based on the heterogenous ABCG1 expression pattern: high risk (High/Low), moderate risk (High/High or Low/Low), and low risk (Low/High). This ABCG1-based risk stratification could distinguish the different OS and RFS in patients with HCC. Multivariate Cox regression analysis indicated that ABCG1 high risk was an independent predictor of poor RFS (P = 0.015).
High nuclear ABCG1 expression indicates poor prognosis in patients with HCC. Asymmetric distribution of ABCG1 in the nucleus and cytoplasm may have an important role in tumor recurrence.
三磷酸腺苷结合盒转运体 G1（ABCG1）调节细胞胆固醇稳态，在肿瘤免疫中发挥重要作用。然而，对于肝细胞癌（HCC），ABCG1 的作用尚未得到研究。因此，本研究旨在评估 ABCG1 在 HCC 中的预后价值和临床病理意义。
纳入 104 例成人 HCC 患者，通过免疫组织化学法检测配对 HCC 标本中 ABCG1 的表达。根据 ABCG1 表达对所有患者进行分层，采用 Kaplan-Meier 分析比较总生存期（OS）和无复发生存期（RFS），并采用 Cox 回归分析确定肿瘤复发的独立预测因素。
与匹配的肿瘤旁组织相比，HCC 样本中观察到 ABCG1 的上调。核 ABCG1 高表达患者的 OS 和 RFS 较低（P=0.012 和 P=0.020）。核 ABCG1 高表达与肿瘤较大（P=0.004）和肿瘤复发（P=0.027）相关。尽管 ABCG1 在细胞质中表达，但细胞质表达不能预测 HCC 患者的预后。根据 ABCG1 的异质性表达模式建立了一种新的分层模式：高风险（High/Low）、中风险（High/High 或 Low/Low）和低风险（Low/High）。这种基于 ABCG1 的风险分层可以区分 HCC 患者不同的 OS 和 RFS。多变量 Cox 回归分析表明，ABCG1 高风险是 RFS 不良的独立预测因素（P=0.015）。
核 ABCG1 高表达提示 HCC 患者预后不良。ABCG1 在核和细胞质中的不对称分布可能在肿瘤复发中起重要作用。
