Nor Farhah Husna Mohd, Abdullah Shakila, Ibrahim Zaharah, Nor Muhamad Hanif Md, Osman Mohd Isa, Al Farraj Dunia A, AbdelGawwad Mohamed Ragab, Kamyab Hesam
Department of Physics and Chemistry, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, Pagoh Education Hub, Pagoh, 84600, Muar, Johor, Malaysia.
Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia.
Bioprocess Biosyst Eng. 2023 Mar;46(3):381-391. doi: 10.1007/s00449-022-02749-1. Epub 2022 Jul 2.
An effective biosurfactant producer and extremophiles bacteria, Bacillus cereus KH1, was isolated from textile effluent and the biosurfactant was produced using molasses as the sole carbon source. Growth parameters such as pH, temperature, salinity and concentration of molasses were optimised for decolourising the textile effluent with 24-h incubation. The biosurfactant property of B. cereus KH1 was evaluated based on haemolytic activity, oil displacement technique, drop-collapsing test and emulsification index. The results of the produced biosurfactant showed a positive reaction in haemolytic activity, oil displacement technique, drop-collapsing test and exhibiting a 67% emulsification index. The cell-free broth was stable in 40 °C pH 7, 7% salinity and 7% molasses. Thin-Layer Chromatography and Fourier Transform Infrared Spectroscopy analysis revealed that the biosurfactant was a lipopeptide with a yield 2.98 g L. These findings proved the synergistic action of B. cereus KH1 with lipopeptide biosurfactant may accelerated the decolourisation efficiency to 87%.
从纺织废水中分离出一种高效的生物表面活性剂产生菌和嗜极菌蜡样芽孢杆菌KH1，并以糖蜜作为唯一碳源生产生物表面活性剂。对pH、温度、盐度和糖蜜浓度等生长参数进行了优化，以便在24小时培养条件下使纺织废水脱色。基于溶血活性、油置换技术、液滴塌陷试验和乳化指数对蜡样芽孢杆菌KH1的生物表面活性剂特性进行了评估。所产生的生物表面活性剂的结果在溶血活性、油置换技术、液滴塌陷试验中显示出阳性反应，乳化指数为67%。无细胞肉汤在40℃、pH 7、7%盐度和7%糖蜜条件下稳定。薄层色谱和傅里叶变换红外光谱分析表明，该生物表面活性剂是一种脂肽，产量为2.98 g/L。这些发现证明蜡样芽孢杆菌KH1与脂肽生物表面活性剂的协同作用可能将脱色效率提高到87%。
