Ocean College, Zhoushan Campus, Zhejiang University, Zhoushan, 316021, China.
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China.
Phytochemistry. 2022 Sep;201:113292. doi: 10.1016/j.phytochem.2022.113292. Epub 2022 Jun 30.
Marine-derived actinomycetes from the genus Streptomycete have a huge potential for the production of metabolites with structural and bioactive uniqueness and diversity. This study described the isolation and structural elucidation of twenty metabolites, including seven previously unreported compounds galbonolide H, galbonolide I, streptophenylpropionic acid A, treptophenylpropyl ester A, streptophenvaleramide A, seco-geldanamycin A and streptorapamycin A, from the marine-associated Streptomycete sp. ZZ1944. Structures of the isolated compounds were elucidated by a combination of extensive NMR spectroscopic analyses, HRESIMS data, optical rotation and ECD calculations. The structure of galbonolide H was also confirmed by a single crystal X-ray diffraction. Both autolytimycin and seco-geldanamycin A showed potent activity against the proliferation of glioma, lung cancer, colorectal cancer and breast cancer cells. Autolytimycin blocked cell cycle of glioma cells and seco-geldanamycin A induced apoptosis of glioma cells.
海洋来源的链霉菌属放线菌具有产生结构和生物活性独特和多样化的代谢产物的巨大潜力。本研究描述了从海洋相关链霉菌属 sp. ZZ1944 中分离和结构解析的二十种代谢产物，包括七种以前未报道的化合物 galbonolide H、galbonolide I、streptophenylpropionic acid A、treptophenylpropyl ester A、streptophenvaleramide A、seco-geldanamycin A 和 streptorapamycin A。通过广泛的 NMR 光谱分析、HRESIMS 数据、旋光和 ECD 计算相结合，确定了分离化合物的结构。galbonolide H 的结构也通过单晶 X 射线衍射得到证实。autolytimycin 和 seco-geldanamycin A 均显示出对神经胶质瘤、肺癌、结直肠癌和乳腺癌细胞增殖的强烈抑制活性。Autolytimycin 阻断神经胶质瘤细胞的细胞周期，而 seco-geldanamycin A 诱导神经胶质瘤细胞凋亡。
