Yi Ke-Xin, Xie Qing-Yi, Ma Qing-Yun, Yang Li, Dai Hao-Fu, Zhao You-Xing, Hao Yu-E
Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.
Haikou Key Laboratory for Research and Utilization of Tropical Natural Products & National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Fitoterapia. 2024 Mar;173:105814. doi: 10.1016/j.fitote.2023.105814. Epub 2023 Dec 30.
Four new ansamycin derivatives, named 1,19-epithio-geldanamycin A (1), 17-demethoxylherbimycin H (2), herbimycin M (3), and seco-geldanamycin B (4), together with eight known ansamycin analogues (5-12) were isolated from the solid fermentation of marine-derived actinomycete Streptomyces sp. ZYX-F-97. The structures of new compounds were elucidated by extensive spectroscopic analysis as well as nuclear magnetic resonance (NMR) and electronic circular dichroism (ECD) calculations. All the compounds were assayed for their antibacterial activity. Among them, compounds 4, 8, and 12 exhibited remarkable inhibition against Listeria monocytogenes with minimum inhibitory concentrations (MIC) values ranging from 8 μg·mL to 64 μg·mL, and displayed moderate inhibition against methicillin-resistant Staphylococcus aureus (MRSA) with MIC value of 64 μg·mL. Compounds 4, 8, 9, and 12 showed moderate inhibition activities against both Staphylococcus aureus and Bacillus subtilis with MIC values ranging from 32 μg·mL to 128 μg·mL.
从海洋来源的放线菌链霉菌属ZYX-F-97的固体发酵产物中分离得到4个新的安莎霉素衍生物，分别命名为1,19-表硫基格尔德霉素A（1）、17-去甲氧基赫比霉素H（2）、赫比霉素M（3）和开环格尔德霉素B（4），以及8个已知的安莎霉素类似物（5-12）。通过广泛的光谱分析以及核磁共振（NMR）和电子圆二色光谱（ECD）计算确定了新化合物的结构。对所有化合物进行了抗菌活性测试。其中，化合物4、8和12对单核细胞增生李斯特菌表现出显著抑制作用，最低抑菌浓度（MIC）值为8 μg·mL至64 μg·mL，对耐甲氧西林金黄色葡萄球菌（MRSA）表现出中等抑制作用，MIC值为64 μg·mL。化合物4、8、9和12对金黄色葡萄球菌和枯草芽孢杆菌均表现出中等抑制活性，MIC值为32 μg·mL至128 μg·mL。
