Valles Gia, Huebschman Jessica L, Chow Elsbeth, Kelly Corinne, Guo Yuhong, Smith Laura N
Department of Neuroscience and Experimental Therapeutics, Texas A&M University Health Science Center, Bryan, TX, United States.
Texas A&M Institute for Neuroscience, Texas A&M University, College Station, TX, United States.
Front Behav Neurosci. 2022 Jun 15;16:880845. doi: 10.3389/fnbeh.2022.880845. eCollection 2022.
Intravenous self-administration (IVSA) is a behavioral method of voluntary drug intake in animal models which is used to study the reinforcing effects of drugs of abuse. It is considered to have greater face validity in the study of substance use and abuse than other assays, and thus, allows for valuable insight into the neurobiological basis of addiction, and the development of substance abuse disorders. The technique typically involves surgically inserting a catheter into the jugular vein, which enables the infusion of drug solution after the performance of a desired operant behavior. Two nose- poke ports or levers are offered as manipulanda and are randomly assigned as active (reinforced) or inactive (non-reinforced) to allow for the examination of discrimination in the assessment of learning. Here, we describe our methodological approach to this assay in a mouse model, including construction and surgical implantation of a jugular vein catheter, set up of operant chambers, and considerations during each phase of the operant task.
静脉自我给药（IVSA）是动物模型中自愿摄入药物的一种行为方法，用于研究滥用药物的强化作用。与其他检测方法相比，它在物质使用和滥用研究中被认为具有更高的表面效度，因此，能够为成瘾的神经生物学基础以及物质滥用障碍的发展提供有价值的见解。该技术通常包括通过手术将导管插入颈静脉，这使得在执行期望的操作性行为后能够输注药物溶液。提供两个鼻戳端口或杠杆作为操作装置，并随机分配为活动（强化）或非活动（非强化），以便在学习评估中检查辨别能力。在这里，我们描述了在小鼠模型中进行该检测的方法，包括颈静脉导管的构建和手术植入、操作性实验箱的设置以及操作性任务各阶段的注意事项。
