Zheng Tianfei, Zhang Qianying, Wu Qiaoyin, Li Dongliang, Wu Xinying, Li Pinhe, Zhou Quanwei, Cai Wen, Zhang Juan, Du Guocheng
School of Biotechnology, Jiangnan University, Wuxi, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Front Microbiol. 2022 Jun 17;13:911791. doi: 10.3389/fmicb.2022.911791. eCollection 2022.
Metabolic activity of the microbial community greatly affects the quality of cigar tobacco leaves (CTLs). To improve the quality of CTLs, two extrinsic microbes ( sp. 1H8 and 3B2) were inoculated into CTLs. The quality of CTLs were significantly improved after fermentation. The content of solanone, 6-methyl-5-hepten-2-one, benzeneacetic acid, ethyl ester, cyclohexanone, octanal, acetophenone, and 3,5,5-trimethyl-2-cyclohexen-1-one were significantly increased after inoculated sp. 1H8. The inoculation of sp. 1H8 enhanced the normal evolutionary trend of bacterial community. The content of trimethyl-pyrazine, 2,6-dimethyl-pyrazine, and megastigmatrienone were significantly increased after inoculated 3B2. The inoculation of 3B2 completely changed the original bacterial community. Network analysis revealed that was negatively correlated with , positively correlated with , and had significant correlations with many volatile flavor compounds. This work may be helpful for improving fermentation product quality by regulating microbial community, and gain insight into the microbial ecosystem.
微生物群落的代谢活动极大地影响雪茄烟叶（CTLs）的品质。为提高CTLs的品质，将两种外源微生物（菌株1H8和3B2）接种到CTLs中。发酵后CTLs的品质显著提高。接种菌株1H8后，茄尼酮、6-甲基-5-庚烯-2-酮、苯乙酸乙酯、环己酮、辛醛、苯乙酮和3,5,5-三甲基-2-环己烯-1-酮的含量显著增加。接种菌株1H8增强了细菌群落的正常演化趋势。接种3B2后，三甲基吡嗪、2,6-二甲基吡嗪和巨豆三烯酮的含量显著增加。接种3B2完全改变了原有的细菌群落。网络分析表明， 与 呈负相关，与 呈正相关，且与许多挥发性风味化合物具有显著相关性。这项工作可能有助于通过调节微生物群落来提高发酵产品质量，并深入了解微生物生态系统。
