Roy Peter J
Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
MicroPubl Biol. 2022 Jun 10;2022. doi: 10.17912/micropub.biology.000587. eCollection 2022.
Unsupervised Uniform Manifold Approximation and Projection (UMAP) plots of single cell sequencing data from synchronized larvae yield tissue-specific data clusters, some of which are plotted as elongated archipelagos. These archipelagos likely represent a single cell type. I show that the pharyngeal archipelagos express a myriad of asynchronous temporally regulated genes, which likely accounts for their elongated topology. With one archipelago, I show that there is a high correlation between a) the base pair distance between the binding sites of an archipelago-specific transcription factor (HLH-6) and the transcriptional start site of the targeted genes and b) the timing of peak gene expression of those genes that are expressed in an archipelago-specific manner. Despite the correlation being made with only four genes, it prompts the hypothesis that the physical distance between a transcription factor and the relevant transcription start site may be an important factor in determining the temporal onset of transcription and transcript abundance.
来自同步化幼虫的单细胞测序数据的无监督均匀流形近似与投影（UMAP）图产生了组织特异性数据簇，其中一些被绘制为细长的群岛状。这些群岛状结构可能代表一种单一的细胞类型。我发现咽群岛表达了大量异步的时间调控基因，这可能解释了它们细长的拓扑结构。对于一个群岛，我发现：a）群岛特异性转录因子（HLH-6）的结合位点与靶向基因的转录起始位点之间的碱基对距离，与b）以群岛特异性方式表达的那些基因的峰值基因表达时间之间存在高度相关性。尽管仅与四个基因进行了相关性分析，但这引发了一个假设，即转录因子与相关转录起始位点之间的物理距离可能是决定转录时间起始和转录本丰度的一个重要因素。
