Mango S E, Lambie E J, Kimble J
Laboratory of Molecular Biology, University of Wisconsin-Madison 53706, USA.
Development. 1994 Oct;120(10):3019-31. doi: 10.1242/dev.120.10.3019.
In the 4-cell Caenorhabditis elegans embryo, two blastomeres are destined to generate pharyngeal cells, each by a distinct developmental strategy: one pathway is inductive, while the other is autonomous. Here, we identify the pha-4 locus. In animals lacking pha-4 activity, an early step in pharyngeal organogenesis is blocked: no pharyngeal primordium is formed and differentiated pharyngeal cells are absent. Most other tissues are generated normally in pha-4 mutants, including cells related to pharyngeal cells by cell lineage and position. Thus, pha-4 activity is required to form the pharyngeal primordium. We propose that pha-4 marks a convergence of the inductive and autonomous pathways of pharyngeal development and suggest that establishment of pharyngeal organ identity is a crucial step for pharyngeal organogenesis.
在四细胞期的秀丽隐杆线虫胚胎中，两个卵裂球注定要通过不同的发育策略产生咽部细胞：一条途径是诱导性的，而另一条是自主性的。在此，我们鉴定出pha-4基因座。在缺乏pha-4活性的动物中，咽部器官发生的早期步骤被阻断：没有形成咽部原基，也没有分化的咽部细胞。pha-4突变体中的大多数其他组织正常产生，包括通过细胞谱系和位置与咽部细胞相关的细胞。因此，形成咽部原基需要pha-4活性。我们提出pha-4标志着咽部发育的诱导性和自主性途径的汇聚，并表明咽部器官身份的确立是咽部器官发生的关键步骤。
