Bhatia Mohit, Singh Ravi Indrapal, Rani Diksha, Rekha U Sasi, Rohilla Ranjana, Omar Balram Ji, Gupta Pratima
Department of Microbiology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.
Department of Microbiology, Sri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun, Uttarakhand, India.
J Pharm Bioallied Sci. 2022 Jan-Mar;14(1):52-55. doi: 10.4103/jpbs.jpbs_651_21. Epub 2022 May 19.
To generate preliminary data about comparative evaluation of two automated ID/AST systems and Mikrolatest kit in determining colistin susceptibility of carbapenem-resistant spp.
Twenty-three carbapenem-resistant and and two carbapenem-sensitive multidrug-resistant isolates obtained from various clinical samples of inpatients were included in the study. Species-level identification and antibiotic susceptibility testing (AST) of test isolates was performed using BD phoenix and MicroScan WalkAway 96 Plus automated systems. Identity was reconfirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Additional colistin susceptibility testing was performed using Mikrolatest MIC colistin susceptibility testing kit (reference method).
Results showed that 16% isolates (27.3% [3/11] and 7.1% [1/14] ) exhibited colistin resistance by the reference method. While the categorical agreement between BD Phoenix M50 ID/AST system and reference test w. r. t colistin susceptibility results was 100% and 92.9% for & , respectively, it was much lower between MicroScan WalkAway 96 plus ID/AST system and the latter. Almost perfect agreement (96%; kappa: 0.834) was observed between BD Phoenix M50 system and reference method.
The results of this study are preliminary and cannot be generalized. Multicentric studies with large sample sizes should be conducted throughout the country to gain a deeper understanding of the subject under consideration.
生成关于两种自动化鉴定/药敏试验（ID/AST）系统与Mikrolatest试剂盒在确定耐碳青霉烯类菌株对黏菌素敏感性方面的比较评估的初步数据。
本研究纳入了从住院患者的各种临床样本中分离出的23株耐碳青霉烯类菌株和2株耐多药且对碳青霉烯类敏感的菌株。使用BD phoenix和MicroScan WalkAway 96 Plus自动化系统对测试菌株进行菌种水平鉴定和抗生素敏感性试验（AST）。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）再次确认菌株身份。使用Mikrolatest MIC黏菌素敏感性试验试剂盒（参考方法）进行额外的黏菌素敏感性试验。
结果显示，通过参考方法，16%的菌株（分别为27.3%[3/11]和7.1%[1/14]）表现出对黏菌素耐药。BD Phoenix M50 ID/AST系统与参考试验在黏菌素敏感性结果方面的分类一致性分别为100%和92.9%，而MicroScan WalkAway 96 plus ID/AST系统与后者之间的一致性则低得多。BD Phoenix M50系统与参考方法之间观察到几乎完美的一致性（96%；kappa值：0.834）。
本研究结果是初步的，不能一概而论。应在全国范围内开展大样本量的多中心研究，以更深入地了解所研究的主题。
