Matsuzaki Etsuko, Hirose Haruna, Matsumoto Kazuma, Matsumoto Noriyoshi, Fujimasa Seishiro, Hatakeyama Junko, Anan Hisashi
Section of Operative Dentistry and Endodontology, Department of Odontology, Fukuoka Dental College, Fukuoka, Japan.
Oral Medicine Research Center, Fukuoka Dental College, Fukuoka, Japan.
J Dent Sci. 2022 Jul;17(3):1232-1237. doi: 10.1016/j.jds.2021.12.006. Epub 2021 Dec 18.
BACKGROUND/PURPOSE: Regarding root-end filling materials in apical surgery, sealing ability and biocompatibility are useful for treatment. Angiogenesis, which occurs in the process of periapical wound healing, is closely related to bone formation. In this study, we investigated the effects of root-end filling materials on vascular endothelial cell proliferation and angiogenesis.
Mineral trioxide aggregate (MTA), 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl borane (4-META/MMA-TBB) resin, Super EBA, and CS-BG-multi, bioactive glass-related materials, were used. After curing, each material was soaked in a medium for 1 or 7 days, and then cultured for 1-7 days to investigate the effects on human umbilical vein endothelial cell (HUVEC) proliferation, angiogenesis, and vascular endothelial growth factor receptors (VEGFRs) mRNA expression.
In the 1-day soaked sample, there was significantly less proliferation in MTA and Super EBA on day 7 of culture. In the 7-day soaked sample, there was significantly less proliferation in Super EBA and CS-BG-multi on day 7 of culture. Tube formation was significantly high in MTA in both the 1-day and 7-day soaked samples, significantly high in SB in the 1-day soaked sample, and significantly low in Super EBA in both the 1-day and 7-day soaked samples. CS-BG-multi was comparable to the control. VEGFR-1 and VEGFR-2 mRNA expressions showed an upward trend in MTA, and a trend similar to the control in SB.
MTA and 4-META/MMA-TBB resin had a higher pro-angiogenic effect while Super EBA had a less pro-angiogenic effect. CS-BG-multi had low toxicity on tube formation of HUVEC.
背景/目的：关于根尖手术中的根尖倒充填材料，封闭能力和生物相容性对治疗很有用。根尖周伤口愈合过程中发生的血管生成与骨形成密切相关。在本研究中，我们研究了根尖倒充填材料对血管内皮细胞增殖和血管生成的影响。
使用了三氧化矿物凝聚体（MTA）、4-甲基丙烯酰氧基乙基偏苯三酸酐/甲基丙烯酸甲酯-三正丁基硼烷（4-META/MMA-TBB）树脂、Super EBA以及CS-BG-multi（生物活性玻璃相关材料）。固化后，每种材料在培养基中浸泡1天或7天，然后培养1至7天，以研究其对人脐静脉内皮细胞（HUVEC）增殖、血管生成和血管内皮生长因子受体（VEGFRs）mRNA表达的影响。
在浸泡1天的样本中，培养第7天时MTA和Super EBA中的细胞增殖明显较少。在浸泡7天的样本中，培养第7天时Super EBA和CS-BG-multi中的细胞增殖明显较少。在浸泡1天和7天的样本中，MTA的管形成均显著较高，浸泡1天的样本中SB的管形成显著较高，浸泡1天和7天的样本中Super EBA的管形成均显著较低。CS-BG-multi与对照组相当。VEGFR-1和VEGFR-2 mRNA表达在MTA中呈上升趋势，在SB中呈与对照组相似的趋势。
MTA和4-META/MMA-TBB树脂具有较高的促血管生成作用，而Super EBA的促血管生成作用较小。CS-BG-multi对HUVEC的管形成毒性较低。
