Vansana Phanthavong, Kakura Kae, Taniguchi Yusuke, Egashira Kei, Matsuzaki Etsuko, Tsutsumi Takashi, Kido Hirofumi
Section of Oral Implantology, Department of Oral Rehabilitation, Fukuoka Dental College, Fukuoka, Japan.
Section of Operative Dentistry and Endodontology, Department of Odontology, Fukuoka Dental College, Fukuoka, Japan.
J Dent Sci. 2022 Jul;17(3):1225-1231. doi: 10.1016/j.jds.2021.12.003. Epub 2021 Dec 20.
BACKGROUND/PURPOSE: 5' Adenosine monophosphate-activated protein kinase (AMPK) is known as an enzyme that maintains intracellular homeostasis and has various biological activity. The purpose of this study is evaluation effect of AMPK activation on implant prognosis.
MATERIALS & METHODS: MC3T3-E1 osteoblast-like cells were cultured on titanium using a 24-well plate. The experimental group was divided into the following 3 groups: (1) the normal culture group (control group), (2) the osteogenic induction group, and (3) the osteogenic induction + AMPK activation group. The cell counts were measured; real-time PCR was used to assess the expression of ALP and Osterix as osteogenic related genes at Day 0,7,14 and 21 after experiments. Additionally, ALP activity and calcification were assessed.
The results of the real-time PCR assessments revealed that the expression of ALP, which is a marker for the initial stages of calcification, was significantly increased by AMPK activation compared to the normal culture or osteogenic induction. A significant increase was also observed in the expression of Osterix, which is a marker for the later stages of calcification. Because significant increases were observed in ALP activity and calcification potential, this suggested that AMPK activation could elicit an increase in osteoblast calcification potential.
AMPK activation promotes implant peripheral osteoblast differentiation and maturation and enhances calcification. Our results suggest that AMPK activation may help to maintain implant stability.
背景/目的：5'-单磷酸腺苷激活蛋白激酶（AMPK）是一种维持细胞内稳态并具有多种生物学活性的酶。本研究的目的是评估AMPK激活对种植体预后的影响。
使用24孔板在钛片上培养MC3T3-E1成骨样细胞。实验组分为以下3组：（1）正常培养组（对照组），（2）成骨诱导组，（3）成骨诱导+AMPK激活组。进行细胞计数；在实验后的第0、7、14和21天，使用实时PCR评估作为成骨相关基因的碱性磷酸酶（ALP）和osterix的表达。此外，评估ALP活性和钙化情况。
实时PCR评估结果显示，与正常培养组或成骨诱导组相比，AMPK激活显著增加了作为钙化初始阶段标志物的ALP的表达。作为钙化后期标志物的osterix的表达也显著增加。由于观察到ALP活性和钙化潜力显著增加，这表明AMPK激活可引起成骨细胞钙化潜力的增加。
AMPK激活促进种植体周围成骨细胞的分化和成熟并增强钙化。我们的结果表明，AMPK激活可能有助于维持种植体稳定性。
