Bellinger L L
Am J Physiol. 1987 May;252(5 Pt 2):R938-46. doi: 10.1152/ajpregu.1987.252.5.R938.
Rats with electrolytic or kainic acid (KA) lesions of the dorsomedial hypothalamic nucleus area (DMHA-L) are hypophagic, hypodipsic, and have a reduced body weight (BW) compared with controls. In the present study, male Sprague-Dawley rats received bilateral ibotenic acid (IBO) lesions of the DMHA (3 micrograms in 0.3 microliter) or sham (S) operations. During the next 32 days the IBO DMHA-L rats showed reduced (P less than 0.01) food and water intake, BW, and linear growth (P less than 0.03), although having a normal Lee obesity index. After a 24-h fast both groups became hyperphagic (P less than 0.01) with the DMHA-L group eating the most (P less than 0.01) during the 1st h; lost BW was regained at the same rate. In the absence of food, DMHA-L rats took less (P less than 0.01) water (data normalized) than S rats. During 24 h of water deprivation, both groups ate similar amounts of food (data normalized); following deprivation the groups were hyperdipsic. Both groups increased their food intake when given 300 mg/kg of 2-deoxy-D-glucose, which contrasts rats with electrolytic or KA DMHA-L rats. Both groups decreased their food intake when given cholecystokinin (3 micrograms/kg ip), which contrasts rats with electrolytic DMHA-L. The DMHA-L rats were not deficient in plasma glucose, insulin, growth hormone, or plasma Na+ and K+. Histology revealed many, but not all neurons, were destroyed in the DMN after IBO. The data indicate that IBO, electrolytic, or KA lesions of the DMHA produce similar but not identical physiological changes.
与对照组相比，下丘脑背内侧核区域（DMHA-L）接受电解损伤或 kainic 酸（KA）损伤的大鼠摄食减少、饮水减少且体重减轻。在本研究中，雄性 Sprague-Dawley 大鼠接受双侧 DMHA 的鹅膏蕈氨酸（IBO）损伤（0.3 微升中含 3 微克）或假手术（S）。在接下来的 32 天里，IBO DMHA-L 大鼠的食物和水摄入量、体重及线性生长均降低（P<0.01），尽管其李氏肥胖指数正常。禁食 24 小时后，两组均出现摄食增多（P<0.01），DMHA-L 组在第 1 小时摄食量最多（P<0.01）；体重减轻以相同速率恢复。在无食物情况下，DMHA-L 大鼠饮水比 S 大鼠少（P<0.01）（数据已标准化）。在 24 小时禁水期间，两组进食量相似（数据已标准化）；禁水后两组均出现饮水增多。给予 300mg/kg 的 2-脱氧-D-葡萄糖时，两组均增加食物摄入量，这与接受电解损伤或 KA 损伤的 DMHA-L 大鼠不同。给予胆囊收缩素（3 微克/千克腹腔注射）时，两组均减少食物摄入量，这与接受电解损伤的 DMHA-L 大鼠不同。DMHA-L 大鼠的血浆葡萄糖、胰岛素、生长激素或血浆 Na+和 K+均无缺乏。组织学检查显示，IBO 损伤后 DMN 中有许多（但并非所有）神经元被破坏。数据表明，DMHA 的 IBO、电解或 KA 损伤产生相似但不完全相同的生理变化。
