Nealon D A, Rej R
Anal Biochem. 1987 Feb 15;161(1):64-9. doi: 10.1016/0003-2697(87)90652-x.
A scheme for the quantitative detection of aspartate aminotransferase isoenzymes and multiple forms after electrophoretic separation is described. Glutamate generated from the aminotransferase reaction is quantitated by using the glutamate dehydrogenase/diaphorase-coupled enzyme system to form a formazan dye. Product inhibition of aspartate aminotransferase by oxaloacetate is prevented by including oxaloacetate decarboxylase in the overlay reagent. Results compare favorably with those of an immunochemical precipitation procedure. The method can also be used to detect quantitatively subforms and atypical forms (genetic variants, immunoglobulin-enzyme complexes) of aspartate aminotransferase.
本文描述了一种电泳分离后定量检测天冬氨酸氨基转移酶同工酶和多种形式的方案。利用谷氨酸脱氢酶/黄递酶偶联酶系统将氨基转移酶反应生成的谷氨酸定量，以形成甲臜染料。通过在覆盖试剂中加入草酰乙酸脱羧酶，可防止草酰乙酸对天冬氨酸氨基转移酶的产物抑制。结果与免疫化学沉淀法相比具有优势。该方法还可用于定量检测天冬氨酸氨基转移酶的亚型和非典型形式（遗传变异体、免疫球蛋白-酶复合物）。
