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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1968 Jan;106(2):401-12. doi: 10.1042/bj1060401.

The subcellular distributions of glutamate decarboxylase and aspartate transaminase were studied in rat and guinea-pig brain. 2. Glutamate decarboxylase is localized in the synaptosome fraction. The mean density of the particles containing the enzyme is slightly greater than those derived from cholinergic neurones, though overlap is substantial. 3. The enzyme is readily released from synaptosomes by hypo-osmotic treatment, but in the presence of Ca(2+), Na(+) and K(+) it sediments with particulate material. 4. The release and binding of the enzyme to membrane fractions by Ca(2+) were investigated. 5. Aspartate transaminase is present in brain as two isoenzymes with different kinetic properties. One isoenzyme is associated with the cytoplasm and the other with mitochondria.

研究了大鼠和豚鼠脑中谷氨酸脱羧酶和天冬氨酸转氨酶的亚细胞分布。2. 谷氨酸脱羧酶定位于突触体部分。含该酶颗粒的平均密度略高于胆碱能神经元来源的颗粒，尽管重叠部分很大。3. 通过低渗处理，该酶很容易从突触体中释放出来，但在Ca(2+)、Na(+)和K(+)存在的情况下，它会与颗粒物质一起沉淀。4. 研究了Ca(2+)对该酶向膜部分的释放和结合情况。5. 脑中的天冬氨酸转氨酶以两种具有不同动力学性质的同工酶形式存在。一种同工酶与细胞质相关，另一种与线粒体相关。

Biochem J. 1968 Jan;106(2):401-12. doi: 10.1042/bj1060401.

The subcellular distributions of glutamate decarboxylase and aspartate transaminase were studied in rat and guinea-pig brain. 2. Glutamate decarboxylase is localized in the synaptosome fraction. The mean density of the particles containing the enzyme is slightly greater than those derived from cholinergic neurones, though overlap is substantial. 3. The enzyme is readily released from synaptosomes by hypo-osmotic treatment, but in the presence of Ca(2+), Na(+) and K(+) it sediments with particulate material. 4. The release and binding of the enzyme to membrane fractions by Ca(2+) were investigated. 5. Aspartate transaminase is present in brain as two isoenzymes with different kinetic properties. One isoenzyme is associated with the cytoplasm and the other with mitochondria.

研究了大鼠和豚鼠脑中谷氨酸脱羧酶和天冬氨酸转氨酶的亚细胞分布。2. 谷氨酸脱羧酶定位于突触体部分。含该酶颗粒的平均密度略高于胆碱能神经元来源的颗粒，尽管重叠部分很大。3. 通过低渗处理，该酶很容易从突触体中释放出来，但在Ca(2+)、Na(+)和K(+)存在的情况下，它会与颗粒物质一起沉淀。4. 研究了Ca(2+)对该酶向膜部分的释放和结合情况。5. 脑中的天冬氨酸转氨酶以两种具有不同动力学性质的同工酶形式存在。一种同工酶与细胞质相关，另一种与线粒体相关。