Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.
Insect Biochem Mol Biol. 2022 Aug;147:103800. doi: 10.1016/j.ibmb.2022.103800. Epub 2022 Jul 1.
Over the last 20 years, advancements in sequencing technologies have highlighted the unique composition of the salivary glands of blood-feeding arthropods. Further biochemical and structural data demonstrated that salivary proteins can disrupt host hemostasis, inflammation and immunity, which favors pathogen transmission. Previously, a Sanger-based sialome of adult Ochlerotatus triseriatus female salivary glands was published based on 731 expressed sequence tag (ESTs). Here, we revisited O. triseriatus salivary gland contents using an Illumina-based sequencing approach of both male and female tissues. In the current data set, we report 10,317 DNA coding sequences classified into several functional classes. The translated transcripts also served as a reference database for proteomic analysis of O. triseriatus female saliva, in which unique peptides from 101 proteins were found. Finally, comparison of male and female libraries allowed for the identification of female-enriched transcripts that are potentially related to blood acquisition and virus transmission.
在过去的 20 年中，测序技术的进步突出了吸血节肢动物唾液腺的独特组成。进一步的生化和结构数据表明，唾液蛋白可以破坏宿主的止血、炎症和免疫功能，从而有利于病原体的传播。此前，曾基于桑格测序法对成年三斑按蚊雌性唾液腺的唾液组进行了研究，基于 731 个表达序列标签（EST）发表了研究结果。在这里，我们使用 Illumina 测序法重新研究了三斑按蚊雌雄组织的唾液腺内容物。在当前的数据集中，我们报告了 10317 个 DNA 编码序列，这些序列被分为几个功能类别。这些转录本也为三斑按蚊雌性唾液的蛋白质组分析提供了参考数据库，在该数据库中发现了 101 种蛋白质的独特肽。最后，雌雄文库的比较鉴定出了可能与吸血和病毒传播有关的雌性特异性富集转录本。
