College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing, China; World Organization for Animal Health (OIE) Reference Lab for Swine Streptococcosis, Nanjing, China; Sanya Institute of Nanjing Agricultural University, Nanjing, China.
Jiangsu Animal Disease Prevention and Control Center, Nanjing, China.
J Glob Antimicrob Resist. 2022 Sep;30:370-376. doi: 10.1016/j.jgar.2022.06.025. Epub 2022 Jul 3.
Polymyxin is considered as one of the 'last lines of defense' for the treatment of multidrug resistant bacteria. Increased use of polymyxin during recent years poses a risk to public health. The purpose of this study was to investigate the carrying situation of the mcr-1 drug-resistance gene in waterfowl in some coastal areas of China from 2019 to 2020.
Fifty-seven isolated avian pathogenic Escherichia coli strains were selected from 493 APEC isolates for whole-genome sequencing. The 24 mcr-1-positive APEC strains were tested for conjugation and genome-wide analysis, including sequence type (ST) analysis, serotype analysis, and drug-resistance gene analysis. Numerous mcr-1-positive E. coli were downloaded from the National Center for Biotechnology Information (NCBI) for comparative genomic analysis.
Antimicrobial susceptibility test results showed that 57 APEC isolates were highly resistant to gentamicin, cefotaxime, and ofloxacin, and 24 mcr-1-positive APEC isolates were resistant to polymyxin. Fourteen isolates of mcr-1-positive APEC plasmids were successfully conjugated to EC600. Both ST156 and ST10 were found in high proportions in human and avian sources through genome-wide analysis; it is worth noting that these two isolates of APEC were detected to contain the bla and bla genes, respectively.
In this study, the epidemiological investigation of the mcr-1 gene was carried out on APEC in some coastal areas of China from 2019 to 2020, and our results have enriched the data on the transmission of APEC isolates carrying the mcr-1 gene in waterfowl.
多黏菌素被认为是治疗多重耐药菌的“最后一道防线”之一。近年来多黏菌素使用量的增加对公共卫生构成了威胁。本研究旨在调查 2019 年至 2020 年中国部分沿海地区水禽中 mcr-1 耐药基因的携带情况。
从 493 株鸭源致病性大肠埃希菌分离株中选择 57 株进行全基因组测序。对 24 株 mcr-1 阳性鸭源致病性大肠埃希菌进行接合实验和全基因组分析，包括序列型（ST）分析、血清型分析和耐药基因分析。从国家生物技术信息中心（NCBI）下载了大量 mcr-1 阳性大肠杆菌进行比较基因组分析。
药敏试验结果显示，57 株鸭源致病性大肠埃希菌对庆大霉素、头孢噻肟和氧氟沙星高度耐药，24 株 mcr-1 阳性鸭源致病性大肠埃希菌对黏菌素耐药。成功将 14 株 mcr-1 阳性鸭源致病性大肠埃希菌质粒接合到 EC600 中。通过全基因组分析发现，ST156 和 ST10 在人类和禽类来源中均有较高比例存在；值得注意的是，这两株鸭源致病性大肠埃希菌均检测到携带 bla 和 bla 基因。
本研究对 2019 年至 2020 年中国部分沿海地区鸭源致病性大肠埃希菌 mcr-1 基因进行了流行病学调查，丰富了水禽携带 mcr-1 基因的鸭源致病性大肠埃希菌传播的数据。
Zotero 插件