Lima Barbieri Nicolle, Nielsen Daniel W, Wannemuehler Yvonne, Cavender Tia, Hussein Ashraf, Yan Shi-Gan, Nolan Lisa K, Logue Catherine M
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, 1802 University Blvd; VMRI #5 Ames, IA, United States of America.
Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
PLoS One. 2017 Mar 6;12(3):e0172997. doi: 10.1371/journal.pone.0172997. eCollection 2017.
Antimicrobial resistance associated with colistin has emerged as a significant concern worldwide threatening the use of one of the most important antimicrobials for treating human disease. Here, we examined a collection (n = 980) of Avian Pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis from the US and internationally for the presence of mcr-1 and mcr-2, genes known to encode colistin resistance. Included in the analysis was an additional set of avian fecal E. coli (AFEC) (n = 220) isolates from healthy birds for comparative analysis. The mcr-1 gene was detected in a total of 12 isolates recovered from diseased production birds from China and Egypt. No mcr genes were detected in the healthy fecal isolates. The full mcr-1 gene from positive isolates was sequenced using specifically designed primers and were compared with sequences currently described in NCBI. mcr-1 positive isolates were also assessed for phenotypic colistin resistance and extended spectrum beta lactam phenotypes and genotypes. This study has identified mcr-1 in APEC isolates dating back to at least 2010 and suggests that animal husbandry practices could result in a potential source of resistance to the human food chain in countries where application of colistin in animal health is practiced.
与黏菌素相关的抗菌药物耐药性已成为全球范围内的一个重大问题，威胁到治疗人类疾病最重要的抗菌药物之一的使用。在此，我们检测了从美国及国际上患有大肠杆菌病的家禽中分离出的980株禽致病性大肠杆菌（APEC），以确定是否存在mcr - 1和mcr - 2基因，这两个基因已知可编码对黏菌素的耐药性。分析中还包括另外一组从健康鸟类中分离出的禽粪便大肠杆菌（AFEC）（n = 220）菌株，用于比较分析。在中国和埃及患病生产禽类中总共检测到12株含有mcr - 1基因的菌株。在健康粪便分离株中未检测到mcr基因。使用专门设计的引物对阳性分离株的完整mcr - 1基因进行测序，并与NCBI目前描述的序列进行比较。还对mcr - 1阳性分离株的黏菌素耐药表型以及超广谱β-内酰胺表型和基因型进行了评估。本研究在至少可追溯到2010年的APEC分离株中鉴定出了mcr - 1，并表明在实施黏菌素用于动物健康的国家，畜牧业做法可能会导致人类食物链潜在的耐药性来源。
