Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou Universitygrid.268415.c, Yangzhou, Jiangsu, People's Republic of China.
Institute of Comparative Medicine, Yangzhou Universitygrid.268415.c, Yangzhou, Jiangsu, People's Republic of China.
Microbiol Spectr. 2022 Apr 27;10(2):e0019622. doi: 10.1128/spectrum.00196-22. Epub 2022 Mar 21.
Emergence of pathogens harboring multiple resistance genes incurs great concerns. Cooccurrence of mobile resistance genes conferring resistance to tigecycline, colistin, and carbapenems in Escherichia coli has not been investigated. This study aimed to characterize three E. coli isolates coharboring (X4), , and . Isolates coharboring (X4), , and were identified and characterized by PCR, Sanger sequencing, antimicrobial susceptibility testing, conjugation assays, Illumina sequencing, nanopore sequencing, and bioinformatic analysis. Three E. coli isolates carrying (X4), , and were identified from pigeons in China. They were resistant to almost all antimicrobials except enrofloxacin. (X4) and could be conjugated into E. coli C600, but was nontransferable in three isolates. Three isolates belonged to sequence type 6775 (ST6775), and clonal dissemination of isolates carrying (X4), , and existed in the pigeon farm. Genetic analysis revealed that mediated by the Tn was located on the chromosome, (X4) was located on the IncFII plasmid, and was located on the IncX3 plasmid. We first characterized the E. coli isolates carrying (X4), , and simultaneously. Relevant measures should be taken to decrease the prevalence of pathogens carrying (X4), , and . Tigecycline and colistin are regarded as vital antimicrobials to treat multidrug-resistant (MDR) bacterial infections, such as that caused by carbapenemase-producing (CPE). Cooccurrence of mobile resistance genes conferring resistance to last-resort antimicrobials in E. coli remains unknown. Here, we characterized E. coli strains coharboring (X4), , and phenotypically and genetically. Resistance genes (X4), , and were located on transposons or plasmids that were mobile genetic elements related to the capture, accumulation, and dissemination of such important resistance genes. The emergence of E. coli isolates carrying (X4), , and highlights the importance of monitoring the coexistence of novel mobile resistance genes in different settings with a One Health approach. Risk of transmission of such MDR pathogens from animals to humans should be evaluated comprehensively.
携带多种耐药基因的病原体的出现引起了极大的关注。尚未研究过同时携带赋予对替加环素、黏菌素和碳青霉烯类抗生素耐药性的移动耐药基因的大肠杆菌。本研究旨在对 3 株同时携带（X4）、、和的大肠杆菌进行鉴定和特征分析。通过 PCR、Sanger 测序、药敏试验、接合试验、Illumina 测序、纳米孔测序和生物信息学分析鉴定和分析了 3 株同时携带（X4）、、和的大肠杆菌分离株。从中国的鸽子中分离出 3 株携带（X4）、、和的大肠杆菌。它们几乎对所有抗菌药物都有耐药性，除了恩诺沙星。（X4）和可以接合到大肠杆菌 C600 中，但在 3 个分离株中不能转移。3 个分离株属于血清型 6775（ST6775），携带（X4）、、和的分离株在鸽子养殖场中存在克隆传播。遗传分析表明，由 Tn 介导的位于染色体上，（X4）位于 IncFII 质粒上，而 位于 IncX3 质粒上。我们首次对同时携带（X4）、、和的大肠杆菌进行了特征分析。应采取相关措施降低携带（X4）、、和的病原体的流行率。替加环素和黏菌素被认为是治疗多药耐药（MDR）细菌感染的重要抗生素，如碳青霉烯酶产生菌（CPE）引起的感染。尚未研究过同时携带赋予对最后手段的抗生素（如替加环素和黏菌素）耐药性的移动耐药基因的大肠杆菌。在这里，我们从表型和遗传上对同时携带（X4）、、和的大肠杆菌菌株进行了特征分析。耐药基因（X4）、、和位于转座子或质粒上，这些移动遗传元件与这些重要耐药基因的捕获、积累和传播有关。携带（X4）、、和的大肠杆菌分离株的出现突出了采用 One Health 方法监测不同环境中新的移动耐药基因共存的重要性。应全面评估此类多药耐药病原体从动物传播给人类的风险。
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