Bessman S P
Anal Biochem. 1987 Mar;161(2):519-23. doi: 10.1016/0003-2697(87)90483-0.
The creatine phosphate shuttle energy transfer mechanism was postulated on the basis of the hexokinase acceptor theory of insulin action. It proposes that the movement of chemical energy from the mitochondrion to the myofibril is in the form of creatine phosphate. This occurs because there are isozymes of creatine phosphokinase bound to the inner membrane of the sarcosome and to the A band of the myofibril. These isozymes have been shown to act as transducers of energy from ATP to creatine phosphate at the translocase site and from creatine phosphate back to ATP at the myofibrillar compartment. Calculations show that there is no significant amount of transformation of creatine phosphate to ATP in the intervening space between the mitochondrion and the myofibril so that, essentially, transport between the oxidative sites and the contractile apparatus is through the creatine phosphate shuttle. There is also evidence that another terminus for this shuttle is the microsome so that muscle activity tends to increase energy supply for protein synthesis.
肌酸磷酸穿梭能量转移机制是基于胰岛素作用的己糖激酶受体理论提出的。该机制认为，化学能从线粒体向肌原纤维的转移是以肌酸磷酸的形式进行的。这是因为肌酸磷酸激酶的同工酶结合在肌粒的内膜和肌原纤维的A带上。这些同工酶已被证明在转位酶位点可作为将能量从ATP转化为肌酸磷酸的转换器，在肌原纤维区又可将能量从肌酸磷酸转化回ATP。计算表明，在介于线粒体和肌原纤维之间的间隙中，肌酸磷酸向ATP的转化量并不显著，因此，基本上，氧化部位和收缩装置之间的运输是通过肌酸磷酸穿梭进行的。还有证据表明，该穿梭的另一个终点是微粒体，因此肌肉活动往往会增加蛋白质合成的能量供应。
