Department of Neurosurgery, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen, 361015, China.
Institute of Analytical Technology and Smart Instruments, Xiamen Huaxia University, Xiamen, 361024, China.
Appl Biochem Biotechnol. 2022 Nov;194(11):5443-5455. doi: 10.1007/s12010-022-04024-1. Epub 2022 Jul 5.
Acute ischemic stroke (AIS) is characterized by a sudden blockage of one of the main arteries supplying blood to the brain, leading to insufficient oxygen and nutrients for brain cells to function properly. Unfortunately, metabolic alterations in the biofluids with AIS are still not well understood. In this study, we performed high-throughput target metabolic analysis on 44 serum samples, including 22 from AIS patients and 22 from healthy controls. Multiple-reaction monitoring analysis of 180 common metabolites revealed a total of 29 metabolites that changed significantly (VIP > 1, p < 0.05). Multivariate statistical analysis unraveled a striking separation between AIS patients and healthy controls. Comparing the AIS group with the control group, the contents of argininosuccinic acid, beta-D-glucosamine, glycerophosphocholine, L-abrine, and L-pipecolic acid were remarkably downregulated in AIS patients. Twenty-nine out of 112 detected metabolites enriched in disturbed metabolic pathways, including aminoacyl-tRNA biosynthesis, glycerophospholipid metabolism, lysine degradation, phenylalanine, tyrosine, and tryptophan biosynthesis metabolic pathways. Collectively, these results will provide a sensitive, feasible diagnostic prospect for AIS patients.
急性缺血性脑卒中（AIS）的特征是大脑主要供血动脉突然阻塞，导致脑细胞无法正常获得氧气和营养物质。不幸的是，目前人们对 AIS 患者生物体液中的代谢变化仍了解甚少。在这项研究中，我们对 44 份血清样本进行了高通量靶向代谢分析，其中包括 22 份 AIS 患者样本和 22 份健康对照者样本。对 180 种常见代谢物进行的多重反应监测分析显示，共有 29 种代谢物发生了显著变化（VIP>1，p<0.05）。多变量统计分析揭示了 AIS 患者和健康对照者之间的明显分离。与对照组相比，AIS 组患者的精氨琥珀酸、β-D-葡糖胺、甘油磷酸胆碱、L-别嘌呤醇和 L-哌可酸含量明显下调。在检测到的 112 种代谢物中，有 29 种富集在失调的代谢途径中，包括氨基酸酰基-tRNA 生物合成、甘油磷脂代谢、赖氨酸降解、苯丙氨酸、酪氨酸和色氨酸生物合成代谢途径。总之，这些结果将为 AIS 患者提供一种敏感、可行的诊断前景。
