Department of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, NO. 8, Gongti South Road, Chaoyang District, Beijing, 100020, China.
Department of Neurology, Peking University Sixth Hospital, Beijing, 100191, China.
Metabolomics. 2021 Feb 8;17(2):24. doi: 10.1007/s11306-021-01774-7.
Ischemic stroke is one of the leading causes of death and adult disability. The incidence of ischemic stroke continues to rise in young adults. This study aimed to provide a comprehensive evaluation of metabolic changes and explore possible mechanisms in young ischemic stroke patients without common risk factors.
This study investigated serum metabolomics in 50 young patients with newly suffered ischemic stroke and 50 age-, sex-, and body mass index-matched healthy controls. Liquid chromatography coupled with a Waters Xevo TQ-S mass spectrometer with an electrospray ionization (ESI) source was used to analyze amino acid or bile acid, and free fatty acid or lipid was analyzed by liquid chromatography coupled with a Qtrap5500 mass spectrometer with an ESI source. The metabolomic data were analyzed by performing a multivariate statistical analysis.
A total of 197 metabolites, including amino acids, bile acids, free fatty acids, and lipids, were identified in all participants. Multivariate models showed significant differences in serum metabolomic patterns between young patients with ischemic stroke and healthy controls. The stroke patients had increased L-methionine, homocysteine, glutamine, uric acid, GCDCA, and PE (18:0/20:4, 16:0/22:5), and decreased levels of L-citrulline, taurine, PC (16:2/22:6, 16:2/20:5, 15:0/18:2), and SM (d18:1/23:0, d20:0/19:1, d18:1/22:0, d16:0/26:1, d16:0/18:0, d16:0/22:1, d18:1/19:1, d16:0/17:1, d16:1/24:1, d18:1/19:0). Based on the identified metabolites, the metabolic pathways of arginine biosynthesis, glycerophospholipid metabolism, and taurine and hypotaurine metabolism were significantly enriched in the young patients with ischemic stroke.
Serum metabolomic patterns were significantly different between young patients with ischemic stroke and healthy controls. Our study is beneficial in providing a further view into the pathophysiology of young patients with ischemic stroke.
缺血性脑卒中是导致死亡和成年人残疾的主要原因之一。年轻人中缺血性脑卒中的发病率持续上升。本研究旨在全面评估代谢变化，并探讨无常见危险因素的年轻缺血性脑卒中患者的可能机制。
本研究纳入了 50 例新发缺血性脑卒中的年轻患者和 50 例年龄、性别和体重指数匹配的健康对照者，采用 Waters Xevo TQ-S 质谱仪（配有电喷雾电离源）检测氨基酸或胆汁酸，采用 AB SCIEX Qtrap5500 质谱仪（配有电喷雾电离源）检测游离脂肪酸或脂质，对其进行液相色谱分析。采用多元统计分析对代谢组学数据进行分析。
在所有参与者中，共鉴定出 197 种代谢物，包括氨基酸、胆汁酸、游离脂肪酸和脂质。多元模型显示，缺血性脑卒中年轻患者和健康对照组之间的血清代谢谱存在显著差异。脑卒中患者的 L-蛋氨酸、同型半胱氨酸、谷氨酰胺、尿酸、GCDCA 和 PE（18:0/20:4、16:0/22:5）水平升高，L-瓜氨酸、牛磺酸、PC（16:2/22:6、16:2/20:5、15:0/18:2）和 SM（d18:1/23:0、d20:0/19:1、d18:1/22:0、d16:0/26:1、d16:0/18:0、d16:0/22:1、d18:1/19:1、d16:0/17:1、d16:1/24:1、d18:1/19:0）水平降低。基于鉴定出的代谢物，精氨酸生物合成、甘油磷脂代谢和牛磺酸及次牛磺酸代谢途径在缺血性脑卒中年轻患者中明显富集。
缺血性脑卒中年轻患者和健康对照组之间的血清代谢谱存在显著差异。本研究有助于进一步了解年轻缺血性脑卒中患者的病理生理学机制。
