Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia/Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha, Saudi Arabia/Blood Products Quality Control and Research Department, National Organization for Research and Control of Biologicals, Cairo, Egypt.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia.
Pak J Pharm Sci. 2022 May;35(3):827-834.
Moringa oleifera plant grows in many countries worldwide and being utilized as a customary medication. The current study aimed to investigate the biological effect of Moringa oleifera leaf extract (MOE) alone or in combination with silver nanoparticles (AgNPs) on colon cancer, microbial cell growth. MOE was utilized in the green synthesis of AgNPs. The characterization of AgNPs was done by UV-Vis-spectrophotometry, X-ray diffraction (XRD) and scanning electron microscopy (SEM). MOE was tested for their sugars, active biomolecules, ROS, protein contents. Results revealed that created AgNPs are about 61 nm in diameter. There were no detectable sugar and protein in MOE, but it contains ROS and active biomolecules. MOE and MOE+AgNPs exerted mild antibacterial action and increased the number of apoptotic cells and p53 protein expression of HT-29 colon cancer cells. MOE and MOE+AgNPs could arrest HT-29 cells at G2/M phase and stimulate splenic cell growth. Both extract preparations showed antioxidant activities. Because MOE and MOE+AgNP stimulated immune cells and activated apoptosis in cancer cells, these preparations can be utilized as anticancer agents.
辣木植物生长在世界上许多国家，并被用作传统药物。本研究旨在探讨辣木叶提取物（MOE）单独或与银纳米粒子（AgNPs）联合对结肠癌、微生物细胞生长的生物学效应。MOE 用于 AgNPs 的绿色合成。AgNPs 的表征通过紫外可见分光光度法、X 射线衍射（XRD）和扫描电子显微镜（SEM）进行。MOE 测试了其糖、活性生物分子、ROS、蛋白质含量。结果表明，所制备的 AgNPs 的直径约为 61nm。MOE 中没有检测到糖和蛋白质，但含有 ROS 和活性生物分子。MOE 和 MOE+AgNPs 对 HT-29 结肠癌细胞表现出轻微的抗菌作用，并增加了凋亡细胞的数量和 p53 蛋白的表达。MOE 和 MOE+AgNPs 可使 HT-29 细胞停滞在 G2/M 期并刺激脾细胞生长。两种提取物均表现出抗氧化活性。由于 MOE 和 MOE+AgNP 刺激免疫细胞并激活癌细胞凋亡，因此这些制剂可用作抗癌药物。
