CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, P. R. China.
University of Chinese Academy of Sciences, Beijing, P. R. China.
Anim Sci J. 2022 Jan-Dec;93(1):e13754. doi: 10.1111/asj.13754.
Limited knowledge is clarified about alterations in the related expression of nutrient chemosensors in the distal small intestine and hindgut under a high-grain (HG) diet in small ruminants. Herein, this study was performed to investigate the expression changes related to nutrient sensing and transport in the ileal and colonic epithelium of goats in response to feeding an HG diet. Twelve Liuyang black goats (similar age and weight) were randomly assigned into two groups: an HG diet (concentrate: hay = 90:10) and a CON diet (concentrate: hay = 55:45). Immunohistochemistry was applied to detect morphological changes in the gut epithelium together with altered expression of chemosensors in the ileum and colon. The results showed that feeding an HG diet increased ileal villus height and depth and induced mucosal sloughing in the colon. The expressions of the nutrient transporters GLUT2, GLUT5, SGLT2, CD36, rBAT, EAAT3, and LAT2 and sensing receptors GPR43 and T1R1 were promoted in the ileum under HG conditions. Moreover, feeding an HG diet also enhanced the expression of GLUT2, SGLT2, CD36, and GPR43 in the colon. These findings indicate that adaptation of the gastrointestinal tract to the HG diet promoted the absorption of glucose, fatty acids, and amino acids in goats.
关于高谷物（HG）饮食对小反刍动物远端小肠和后肠中营养化学感受器相关表达的改变，人们的了解有限。因此，本研究旨在研究高谷物饮食对山羊回肠和结肠上皮中与营养感知和运输相关的表达变化。将 12 只浏阳黑山羊（年龄和体重相似）随机分为两组：高谷物饮食组（精料：干草=90：10）和对照饮食组（精料：干草=55：45）。应用免疫组织化学方法检测肠道上皮形态变化以及回肠和结肠中化学感受器的改变表达。结果表明，高谷物饮食增加了回肠绒毛高度和深度，并诱导了结肠黏膜脱落。在 HG 条件下，营养转运体 GLUT2、GLUT5、SGLT2、CD36、rBAT、EAAT3 和 LAT2 以及感应受体 GPR43 和 T1R1 的表达在回肠中得到促进。此外，高谷物饮食还增强了结肠中 GLUT2、SGLT2、CD36 和 GPR43 的表达。这些发现表明，胃肠道对高谷物饮食的适应促进了山羊对葡萄糖、脂肪酸和氨基酸的吸收。
