School of Biological Sciences, University of Portsmouth, Portsmouth, UK.
Laboratory of Plant and Environmental Biotechnology, Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Biofouling. 2022 May;38(5):521-535. doi: 10.1080/08927014.2022.2094259. Epub 2022 Jul 6.
In the maritime field where biofouling has both economic and environmental impacts, quantification methods of biofilm development are of outstanding importance. Indeed, it is challenging to temporally monitor biofilm formation due to the complexity of the marine ecosystem, common inaccessibility of sampling location and lack of standardized techniques. Here, an artificial polymeric surface was tested and against natural phototrophic biofilms and monoculture biofilms using plate reader fluorescence. The suitability of the developed method was verified using fluorescence microscopy coupled with image analysis - a common quantification technique - demonstrating a strong correlation between the tested methods. The results indicated the efficiency of inherent chlorophyll fluorescence in quantifying undisturbed phototrophic biofilms in field and laboratory conditions using microplate reader. This work demonstrated that the suggested approach is promising for biofilm high-throughput testing, and therefore has the potential to be used in several research and industrial sectors for monitoring phototrophic biofilm development.
在海洋领域，生物污损具有经济和环境双重影响，生物膜发展的定量方法尤为重要。由于海洋生态系统的复杂性，采样地点通常难以进入，且缺乏标准化技术，因此很难对生物膜的形成进行时间监测。在这里，使用平板读数荧光法对人工聚合物表面进行了测试，并与天然光养生物膜和单培养生物膜进行了对比。通过荧光显微镜结合图像分析（一种常用的定量技术）验证了所开发方法的适用性，证明了两种测试方法之间具有很强的相关性。结果表明，固有叶绿素荧光在使用微孔板读数器现场和实验室条件下定量未受干扰的光养生物膜方面非常有效。这项工作表明，所提出的方法有望用于生物膜高通量测试，因此有可能在几个研究和工业领域用于监测光养生物膜的发展。
