Key Laboratory of High Performance Plastics, Ministry of Education, College of Chemistry, Jilin University, Changchun 130012, P. R. China.
Norman Bethune First Hospital, Jilin University, Changchun 130021, P. R. China.
J Mater Chem B. 2022 Jul 20;10(28):5473-5486. doi: 10.1039/d2tb00725h.
Long carbon fiber reinforced polyetheretherketone (LCFRPEEK), a newly developed high-performance composite material, is being investigated as a possible orthopedic implant. However, its inability of angiogenesis and osseointegration after implantation makes it difficult for use as a long-term osteogenic fixation implant, which limits its scope of clinical application. Therefore, we design and construct a multifunctional 3D sponge-like macroporous cryogel to modify sulfonated LCFRPEEK using a cryogelation method based on free radical photopolymerization. The cryogel is mainly composed of graphene oxide-hydroxyapatite (GO-HAP) nanocomposites and gelatin methacrylate/polyethylene glycol diacrylate (GelMA/PEGDA). The results reveal that the multifunctional LCFRPEEK implant shows excellent biocompatibility and osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) due to the incorporation of HAP nanoparticles into GO-HAP nanocomposites. Systematic animal studies further confirm that the multifunctional surface improves the bone remodeling and osseointegration of the LCFRPEEK implant. Additionally, the characteristic 3D sponge-like macroporous structures of cryogels promote the ingrowth and migration of human umbilical vein endothelial cells (HUVECs) and GO in the GO-HAP also boosts HUVEC migration and tube formation showing that they are beneficial for vascularization during osteogenesis. Therefore, the developed 3D sponge-like macroporous GelMA/PEGDA/GO-HAP cryogel fabricated on sulfonated LCFRPEEK implants with enhanced angiogenesis and osseointegration capabilities has great potential for clinical use as an orthopedic implant material.
长碳纤维增强聚醚醚酮（LCFRPEEK）是一种新兴的高性能复合材料，正在被研究作为一种潜在的骨科植入物。然而，其在植入后的血管生成和骨整合能力不足，使其难以作为长期成骨固定植入物使用，从而限制了其临床应用范围。因此，我们设计并构建了一种多功能 3D 海绵状大孔冷冻凝胶，通过自由基光聚合的冷冻凝胶化方法对磺化 LCFRPEEK 进行修饰。该冷冻凝胶主要由氧化石墨烯-羟基磷灰石（GO-HAP）纳米复合材料和甲基丙烯酰化明胶/聚乙二醇二丙烯酸酯（GelMA/PEGDA）组成。结果表明，多功能 LCFRPEEK 植入物由于 HAP 纳米颗粒的掺入到 GO-HAP 纳米复合材料中，表现出优异的生物相容性和大鼠骨髓间充质干细胞（BMSCs）的成骨分化能力。系统的动物研究进一步证实，多功能表面改善了 LCFRPEEK 植入物的骨重塑和骨整合。此外，冷冻凝胶的特征 3D 海绵状大孔结构促进了人脐静脉内皮细胞（HUVECs）和 GO 的向内生长和迁移，GO-HAP 中的 GO 也促进了 HUVEC 的迁移和管形成，表明它们有利于成骨过程中的血管生成。因此，在增强血管生成和骨整合能力的磺化 LCFRPEEK 植入物上制备的具有增强血管生成和骨整合能力的 3D 海绵状大孔 GelMA/PEGDA/GO-HAP 冷冻凝胶具有作为骨科植入物材料的临床应用潜力。
