不动杆菌属菌株GJ70的野生型和突变体对正卤代烷和α,ω-二卤代烷的降解作用
Degradation of n-haloalkanes and alpha, omega-dihaloalkanes by wild-type and mutants of Acinetobacter sp. strain GJ70.
作者信息
Janssen D B, Jager D, Witholt B
出版信息
Appl Environ Microbiol. 1987 Mar;53(3):561-6. doi: 10.1128/aem.53.3.561-566.1987.
Abstract
A 1,6-dichlorohexane-degrading strain of Acinetobacter sp. was isolated from activated sludge. The organism could grow with and quantitatively release halide from 1,6-dichlorohexane, 1,9-dichlorononane, 1-chloropentane, 1-chlorobutane, 1-bromopentane, ethylbromide, and 1-iodopropane. Crude extracts contained an inducible novel dehalogenase that liberated halide from the above compounds and also from 1,3-dichloropropane, 1,2-dibromoethane, and 2-bromoethanol. The latter two compounds were toxic suicide substrates for the organism at concentrations of 10 and 5 microM, respectively. Mutants resistant to 1,2-dibromoethane (3 mM) lacked dehalogenase activity and did not utilize haloalkanes for growth. Mutants resistant to both 1,2-dibromoethane (3 mM) and 2-bromoethanol (30 mM) could no longer oxidize or utilize alcohols and were capable of hydrolytic dehalogenation of 1,2-dibromoethane to ethylene glycol.
摘要
从活性污泥中分离出一株能降解1,6 - 二氯己烷的不动杆菌属菌株。该菌株能够利用1,6 - 二氯己烷、1,9 - 二氯壬烷、1 - 氯戊烷、1 - 氯丁烷、1 - 溴戊烷、溴乙烷和1 - 碘丙烷生长，并定量释放卤化物。粗提物中含有一种可诱导的新型脱卤酶，该酶能从上述化合物以及1,3 - 二氯丙烷、1,2 - 二溴乙烷和2 - 溴乙醇中释放卤化物。后两种化合物分别在10 microM和5 microM的浓度下对该菌株是有毒的自杀底物。对1,2 - 二溴乙烷（3 mM）具有抗性的突变体缺乏脱卤酶活性，不能利用卤代烷烃生长。对1,2 - 二溴乙烷（3 mM）和2 - 溴乙醇（30 mM）都具有抗性的突变体不再能够氧化或利用醇类，并且能够将1,2 - 二溴乙烷水解脱卤生成乙二醇。
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不过严格按照要求，最终译文为：莫拉菌属、不动杆菌属及嗜肺军团菌属。
需注意，如果单纯按照你要求的不添加任何解释或说明只翻译原文，是无法对其中一些可能存在歧义的词进行背景注释的，上述括号内内容只是供你理解翻译思路，实际翻译输出仅为：莫拉菌属、不动杆菌属及嗜肺军团菌属。
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